KCNMB2 Antibody - #DF3862

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Product: KCNMB2 Antibody
Catalog: DF3862
Description: Rabbit polyclonal antibody to KCNMB2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 30 KD; 27kD(Calculated).
Uniprot: Q9Y691
RRID: AB_2836219

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KCNMB2 Antibody detects endogenous levels of total KCNMB2.
RRID:
AB_2836219
Cite Format: Affinity Biosciences Cat# DF3862, RRID:AB_2836219.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2700049B16Rik; 3110031N04Rik; BK channel subunit beta 2; BK channel subunit beta-2; BKbeta2; Calcium activated potassium channel subfamily M subunit beta 2; Calcium activated potassium channel subunit beta 2; Calcium-activated potassium channel; Calcium-activated potassium channel subunit beta-2; Charybdotoxin receptor subunit beta 2; Charybdotoxin receptor subunit beta-2; Hbeta2; Hbeta3; K(VCA)beta-2; K(VCA)beta2; KCMB2_HUMAN; KCNMB 2; Kcnmb2; Large conductance Ca2+ activated K+ channel beta2 subunit; Large conductance calcium activated potassium channel beta 2 subunit; Maxi K channel subunit beta 2; Maxi K channel subunit beta-2; MaxiK channel beta 2 subunit; MGC22431; MGC57945; Potassium large conductance calcium activated channel subfamily M beta member 2; Slo-beta-2; Slobeta2; subfamily M subunit beta-2;

Immunogens

Immunogen:
Uniprot:

Q9Y691(KCMB2_HUMAN) >>Visit HPA database.

Gene(ID):
KCNMB2(10242)
Expression:
Q9Y691 KCMB2_HUMAN:

Expressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.

Sequence:
MFIWTSGRTSSSYRHDEKRNIYQKIRDHDLLDKRKTVTALKAGEDRAILLGLAMMVCSIMMYFLLGITLLRSYMQSVWTEESQCTLLNASITETFNCSFSCGPDCWKLSQYPCLQVYVNLTSSGEKLLLYHTEETIKINQKCSYIPKCGKNFEESMSLVNVVMENFRKYQHFSCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGGVAIVAMVKLTQYLSLLCERIQRINR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Chicken
75
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y691 As Substrate

Site PTM Type Enzyme
Y13 Phosphorylation
T38 Phosphorylation
Y130 Phosphorylation

Research Backgrounds

Function:

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons.

PTMs:

N-glycosylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.

Subunit Structure:

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer.

Family&Domains:

The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus.

Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

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