ARFGEF2 Antibody - #DF3857

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Product: ARFGEF2 Antibody
Catalog: DF3857
Description: Rabbit polyclonal antibody to ARFGEF2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 210 KD; 202kD(Calculated).
Uniprot: Q9Y6D5
RRID: AB_2836214

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Horse(92%), Rabbit(80%), Dog(91%)
Clonality:
Polyclonal
Specificity:
ARFGEF2 Antibody detects endogenous levels of total ARFGEF2.
RRID:
AB_2836214
Cite Format: Affinity Biosciences Cat# DF3857, RRID:AB_2836214.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited); ADP ribosylation factor guanine nucleotide exchange factor 2; ARFGEF 2; ARFGEF2; ARFGEP2; BIG 2; BIG2; Brefeldin A inhibited 2; Brefeldin A inhibited GEP 2; Brefeldin A inhibited guanine nucleotide exchange protein 2; dJ1164I10.1; FLJ23723;

Immunogens

Immunogen:
Uniprot:

Q9Y6D5(BIG2_HUMAN) >>Visit HPA database.

Gene(ID):
ARFGEF2(10564)
Expression:
Q9Y6D5 BIG2_HUMAN:

Expressed in placenta, lung, heart, brain, kidney and pancreas.

Sequence:
MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Pig
91
Dog
91
Rabbit
80
Bovine
56
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6D5 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K17 Acetylation
K22 Acetylation
K22 Ubiquitination
K25 Acetylation
K59 Ubiquitination
T101 Phosphorylation
S107 Phosphorylation
S214 Phosphorylation
S218 Phosphorylation
S227 Phosphorylation
S236 Phosphorylation
S240 Phosphorylation
T243 Phosphorylation
T244 Phosphorylation
S260 Phosphorylation
S276 Phosphorylation
S277 Phosphorylation
S279 Phosphorylation
T281 Phosphorylation
K302 Ubiquitination
S346 Phosphorylation
S348 Phosphorylation
S349 Phosphorylation
K383 Acetylation
T424 Phosphorylation
S569 Phosphorylation
T589 Phosphorylation
S590 Phosphorylation
S614 Phosphorylation
T616 Phosphorylation
S617 Phosphorylation
S620 Phosphorylation
T621 Phosphorylation
S690 Phosphorylation
T691 Phosphorylation
S700 Phosphorylation
K741 Ubiquitination
K794 Acetylation
Y797 Phosphorylation
K808 Ubiquitination
K840 Ubiquitination
T866 Phosphorylation
S875 Phosphorylation
T882 Phosphorylation
S883 Phosphorylation
T885 Phosphorylation
T1007 Phosphorylation
K1010 Ubiquitination
S1015 Phosphorylation
S1024 Phosphorylation
T1029 Phosphorylation
S1064 Phosphorylation
S1067 Phosphorylation
S1079 Phosphorylation
K1167 Ubiquitination
K1171 Ubiquitination
K1181 Ubiquitination
S1498 Phosphorylation
S1499 Phosphorylation
S1511 Phosphorylation
S1513 Phosphorylation
S1514 Phosphorylation
K1517 Ubiquitination
S1520 Phosphorylation
S1525 Phosphorylation
S1528 Phosphorylation
T1531 Phosphorylation
S1534 Phosphorylation
K1536 Ubiquitination
Y1632 Phosphorylation
K1727 Ubiquitination
S1782 Phosphorylation

Research Backgrounds

Function:

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.

PTMs:

In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.

Subcellular Location:

Cytoplasm. Membrane. Golgi apparatus. Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Endosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell projection>Dendrite. Cytoplasmic vesicle. Cell junction>Synapse. Cytoplasm>Cytoskeleton.
Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in placenta, lung, heart, brain, kidney and pancreas.

Subunit Structure:

Homodimer (Probable). Interacts with ARFGEF1/BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with PRKAR1A, PRKAR2A, PRKAR1B, PRKAR2B, PPP1CC, PDE3A, TNFRSF1A, MYCBP and EXOC7. Interacts with GABRB1, GABRB2 and GABRB3 (By similarity).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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