BCAS2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat muscle, using BCAS2 Antibody.

Product:	BCAS2 Antibody
Catalog:	DF3855
Description:	Rabbit polyclonal antibody to BCAS2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	27 KD; 26kD(Calculated).
Uniprot:	O75934
RRID:	AB_2836212

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

BCAS2 Antibody detects endogenous levels of total BCAS2.

RRID:

AB_2836212
Cite Format: Affinity Biosciences Cat# DF3855, RRID:AB_2836212.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bcas2; Breast carcinoma amplified sequence 2; Breast carcinoma-amplified sequence 2; DAM1; DNA amplified in mammary carcinoma 1 protein; MGC7712; Pre mRNA splicing factor SPF27; Pre-mRNA-splicing factor spf27; Snt309; SPF27; SPF27_HUMAN; Spliceosome associated protein amplified in breast cancer; Spliceosome associated protein SPF 27; Spliceosome-associated protein SPF 27;

Immunogens

Immunogen:

Uniprot:

O75934(SPF27_HUMAN) >>Visit HPA database.

Gene(ID):

BCAS2(10286)

Expression:

O75934 SPF27_HUMAN:

Ubiquitously expressed.

Sequence:

O75934 SPF27_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Zebrafish

100

Chicken

100

Rabbit

100

Xenopus

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75934 As Substrate

O75934

Site	PTM Type	Source
A2	Acetylation	Uniprot
T4	Phosphorylation	Uniprot
Y23	Phosphorylation	Uniprot
K47	Ubiquitination	Uniprot
S59	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
K85	Ubiquitination	Uniprot
Y87	Phosphorylation	Uniprot
S93	Phosphorylation	Uniprot
S94	Phosphorylation	Uniprot
K97	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K158	Ubiquitination	Uniprot
K168	Ubiquitination	Uniprot
K177	Ubiquitination	Uniprot
Y193	Phosphorylation	Uniprot
Y207	Phosphorylation	Uniprot
K210	Ubiquitination	Uniprot
K218	Ubiquitination	Uniprot

Research Backgrounds

O75934_SPF27_HUMAN

Function:

Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

Subcellular Location:

Nucleus. Nucleus>Nucleolus.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.

Family&Domains:

Belongs to the SPF27 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

Restrictive clause

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BCAS2 Antibody - #DF3855