BCAS2 Antibody - #DF3855

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Product: BCAS2 Antibody
Catalog: DF3855
Description: Rabbit polyclonal antibody to BCAS2
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 27 KD; 26kD(Calculated).
Uniprot: O75934
RRID: AB_2836212

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
BCAS2 Antibody detects endogenous levels of total BCAS2.
RRID:
AB_2836212
Cite Format: Affinity Biosciences Cat# DF3855, RRID:AB_2836212.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bcas2; Breast carcinoma amplified sequence 2; Breast carcinoma-amplified sequence 2; DAM1; DNA amplified in mammary carcinoma 1 protein; MGC7712; Pre mRNA splicing factor SPF27; Pre-mRNA-splicing factor spf27; Snt309; SPF27; SPF27_HUMAN; Spliceosome associated protein amplified in breast cancer; Spliceosome associated protein SPF 27; Spliceosome-associated protein SPF 27;

Immunogens

Immunogen:

A synthesized peptide derived from human BCAS2, corresponding to a region within the internal amino acids.

Uniprot:

O75934(SPF27_HUMAN) >>Visit HPA database.

Gene(ID):
BCAS2(10286)
Expression:
O75934 SPF27_HUMAN:

Ubiquitously expressed.

Sequence:
MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
92
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

Subcellular Location:

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Family&Domains:

Belongs to the SPF27 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

References

1). Molecular characterization, expression patterns and cellular localization of BCAS2 gene in male Hezuo pig. PeerJ, 2023 (PubMed: 37901468) [IF=2.3]

Application: WB    Species: Pig    Sample:

Figure 6. Expression patterns of Hezuo pig BCAS2 at the mRNA and protein levels. (A) The relative expression levels of BCAS2 mRNA in various tissues at 240 d Hezuo pig. β-actin was used as a reference gene. (B) The relative expression levels of BCAS2 mRNA in Hezuo pig testes at different development stages. (C) Western blot bands of BCAS2 and β-actin in Hezuo pig testes at different development stages. β-actin was used as a loading control. (D) The relative expression levels of BCAS2 protein in Hezuo pig testes at different development stages. The bars represent means ± SD from three independent experiments. **p < 0.01, *p < 0.05, and ns, no significance. 30 d: 30 days old, 120 d: 120 days old, and 240 d: 240 days old.

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