BAIAP2L1 Antibody - #DF3853

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Product: BAIAP2L1 Antibody
Catalog: DF3853
Description: Rabbit polyclonal antibody to BAIAP2L1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 57 KD; 57kD(Calculated).
Uniprot: Q9UHR4
RRID: AB_2836210

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
BAIAP2L1 Antibody detects endogenous levels of total BAIAP2L1.
RRID:
AB_2836210
Cite Format: Affinity Biosciences Cat# DF3853, RRID:AB_2836210.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAI1 associated protein 2 like 1; BAI1 associated protein 2 like protein 1; BAI1-associated protein 2-like protein 1; Baiap2l1; BI2L1_HUMAN; Brain specific angiogenesis inhibitor 1 associated protein 2 like protein 1; Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1; FLJ42275; Insulin receptor tyrosine kinase substrate; IRTKS;

Immunogens

Immunogen:
Uniprot:

Q9UHR4(BI2L1_HUMAN) >>Visit HPA database.

Gene(ID):
BAIAP2L1(55971)
Sequence:
MSRGPEEVNRLTESTYRNVMEQFNPGLRNLINLGKNYEKAVNAMILAGKAYYDGVAKIGEIATGSPVSTELGHVLIEISSTHKKLNESLDENFKKFHKEIIHELEKKIELDVKYMNATLKRYQTEHKNKLESLEKSQAELKKIRRKSQGSRNALKYEHKEIEYVETVTSRQSEIQKFIADGCKEALLEEKRRFCFLVDKHCGFANHIHYYHLQSAELLNSKLPRWQETCVDAIKVPEKIMNMIEEIKTPASTPVSGTPQASPMIERSNVVRKDYDTLSKCSPKMPPAPSGRAYTSPLIDMFNNPATAAPNSQRVNNSTGTSEDPSLQRSVSVATGLNMMKKQKVKTIFPHTAGSNKTLLSFAQGDVITLLIPEEKDGWLYGEHDVSKARGWFPSSYTKLLEENETEAVTVPTPSPTPVRSISTVNLSENSSVVIPPPDYLECLSMGAAADRRADSARTTSTFKAPASKPETAAPNDANGTAKPPFLSGENPFATVKLRPTVTNDRSAPIIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHR4 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
Y37 Phosphorylation P12931 (SRC)
K39 Ubiquitination
Y51 Phosphorylation
T63 Phosphorylation
K84 Ubiquitination
K94 Ubiquitination
K98 Ubiquitination
Y114 Phosphorylation
T118 Phosphorylation
S132 Phosphorylation
S136 Phosphorylation
K146 Ubiquitination
S147 Phosphorylation
S150 Phosphorylation
K155 Ubiquitination
Y156 Phosphorylation P12931 (SRC)
Y163 Phosphorylation P12931 (SRC)
T166 Phosphorylation
K183 Ubiquitination
K234 Ubiquitination
K238 Ubiquitination
T248 Phosphorylation
S251 Phosphorylation
T252 Phosphorylation
S255 Phosphorylation
T257 Phosphorylation
S261 Phosphorylation
Y274 Phosphorylation P12931 (SRC)
T276 Phosphorylation
S278 Phosphorylation
K279 Ubiquitination
S281 Phosphorylation
Y293 Phosphorylation P12931 (SRC)
T294 Phosphorylation
S295 Phosphorylation
S329 Phosphorylation
S331 Phosphorylation
T334 Phosphorylation
T351 Phosphorylation
S354 Phosphorylation
Y380 Phosphorylation
S394 Phosphorylation
S395 Phosphorylation
Y396 Phosphorylation
T397 Phosphorylation
T409 Phosphorylation
T412 Phosphorylation
S414 Phosphorylation
T416 Phosphorylation
S420 Phosphorylation
S422 Phosphorylation
T423 Phosphorylation
Y439 Phosphorylation P12931 (SRC)
S444 Phosphorylation
T494 Phosphorylation
K496 Ubiquitination
R505 Methylation

Research Backgrounds

Function:

May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.

PTMs:

Phosphorylated on tyrosine in response to insulin.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with RAC1. Binds to F-actin. Interacts with FASLG. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U). Interacts with E.coli intimin receptor Tir.

Family&Domains:

The IMD domain is predicted to have a helical structure. It may induce actin bundling and filopodia formation (By similarity).

References

1). Insulin Receptor Substrate p53 Ameliorates High-Glucose-Induced Activation of NF-κB and Impaired Mobility of HUVECs. Biomed Research International, 2021 (PubMed: 33506012) [IF=2.6]

Application: WB    Species: Human    Sample: HUVECs

Figure 1 A high D-glucose concentration affected the expression levels of IRSp53 and gal-3 in HUVECs. (a) The expression levels of IRSp53 and gal-3 in HUVECs treated with D-glucose at different concentration for 96 h were determined by Western blotting using β-actin as a loading control. NG: normal glucose (25 mM); H1: high glucose 1 (40 mM); H2: high glucose 2 (60 mM); H3: high glucose 3 (80 mM). (b) The expression levels of IRSp53 and gal-3 in HUVECs treated with 60 mM D-glucose for different times were determined by Western blotting using β-actin as a loading control. (c) The expression levels of IRSp53 and gal-3 in HUVECs treated with 60 mM D-glucose or 60 mM mannitol for 96 h were determined by Western blotting using β-actin as a loading control. NG: normal glucose (25 mM); HG: high glucose (60 mM); MN: mannitol (60 mM). The values are the mean ± SD of three independent experiments. ∗P < 0.05 vs. the NG group; ∗∗P < 0.01 vs. the NG group; ∗∗∗P < 0.001 vs. the NG group.

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