Product: SLC27A5 Antibody
Catalog: DF3845
Description: Rabbit polyclonal antibody to SLC27A5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 75 KD; 75kD(Calculated).
Uniprot: Q9Y2P5
RRID: AB_2836202

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(91%)
Clonality:
Polyclonal
Specificity:
SLC27A5 Antibody detects endogenous levels of total SLC27A5.
RRID:
AB_2836202
Cite Format: Affinity Biosciences Cat# DF3845, RRID:AB_2836202.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACSB; ACSVL6; BA CoA ligase; BA-CoA ligase; BACS; BAL; Bile acid CoA ligase; Bile acid-CoA ligase; Bile acyl CoA synthetase; Bile acyl-CoA synthetase; Cholate CoA ligase; Cholate--CoA ligase; FACVL3; FATP 5; FATP-5; FATP5; Fatty acid Coenzyme A ligase very long chain 3; Fatty acid transport protein 5; Fatty-acid-coenzyme A ligase; FLJ22987; S27A5_HUMAN; Slc27a5; Solute carrier family 27 (fatty acid transporter) member 5; Solute carrier family 27 member 5; Very long chain acyl CoA synthetase homolog 2; Very long chain acyl CoA synthetase related protein; very long-chain 3; Very long-chain acyl-CoA synthetase homolog 2; Very long-chain acyl-CoA synthetase-related protein; VLACS related; VLACS-related; VLACSR; VLCS H2; VLCS-H2; VLCSH2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y2P5 S27A5_HUMAN:

Predominantly expressed in liver.

Sequence:
MGVRQQLALLLLLLLLLWGLGQPVWPVAVALTLRWLLGDPTCCVLLGLAMLARPWLGPWVPHGLSLAAAALALTLLPARLPPGLRWLPADVIFLAKILHLGLKIRGCLSRQPPDTFVDAFERRARAQPGRALLVWTGPGAGSVTFGELDARACQAAWALKAELGDPASLCAGEPTALLVLASQAVPALCMWLGLAKLGCPTAWINPHGRGMPLAHSVLSSGARVLVVDPDLRESLEEILPKLQAENIRCFYLSHTSPTPGVGALGAALDAAPSHPVPADLRAGITWRSPALFIYTSGTTGLPKPAILTHERVLQMSKMLSLSGATADDVVYTVLPLYHVMGLVVGILGCLDLGATCVLAPKFSTSCFWDDCRQHGVTVILYVGELLRYLCNIPQQPEDRTHTVRLAMGNGLRADVWETFQQRFGPIRIWEVYGSTEGNMGLVNYVGRCGALGKMSCLLRMLSPFELVQFDMEAAEPVRDNQGFCIPVGLGEPGLLLTKVVSQQPFVGYRGPRELSERKLVRNVRQSGDVYYNTGDVLAMDREGFLYFRDRLGDTFRWKGENVSTHEVEGVLSQVDFLQQVNVYGVCVPGCEGKVGMAAVQLAPGQTFDGEKLYQHVRAWLPAYATPHFIRIQDAMEVTSTFKLMKTRLVREGFNVGIVVDPLFVLDNRAQSFRPLTAEMYQAVCEGTWRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Pig
73
Sheep
73
Rabbit
73
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2P5 As Substrate

Site PTM Type Enzyme
K241 Acetylation
S316 Phosphorylation
S501 Phosphorylation
S515 Phosphorylation
Y613 Phosphorylation

Research Backgrounds

Function:

Acyl-CoA synthetase that catalyzes the activation of bile acids via formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine. Both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates. Also exhibits acyl CoA synthetase activity that activates very long-chain fatty acids (VLCFAs) by catalyzing the formation of fatty acyl-CoA. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Exhibits long-chain fatty acids (LCFA) transport activity. Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Microsome. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in liver.

Family&Domains:

Belongs to the ATP-dependent AMP-binding enzyme family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Over-Expression and Prognostic Significance of FATP5, as a New Biomarker, in Colorectal Carcinoma. Frontiers in Molecular Biosciences, 2022 (PubMed: 35155561) [IF=5.0]

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