Product: B3GALT1 Antibody
Catalog: DF3833
Description: Rabbit polyclonal antibody to B3GALT1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 36 KD; 38kD(Calculated).
Uniprot: Q9Y5Z6
RRID: AB_2836190

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
B3GALT1 Antibody detects endogenous levels of total B3GALT1.
RRID:
AB_2836190
Cite Format: Affinity Biosciences Cat# DF3833, RRID:AB_2836190.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3-galactosyltransferase 1; 3-GalTase 1; B3GALT1; B3GT1_HUMAN; Beta 1,3 galactosyltransferase 1; Beta 1,3 GalTase 1; Beta 3 galt1; Beta-1; beta3Gal T1; Beta3Gal-T1; Beta3GalT1; UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 1; UDP galactose:beta N acetyl glucosamine beta 1,3 galactosyltransferase 1; UDP-galactose:beta-N-acetyl-glucosamine-beta-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y5Z6 B3GT1_HUMAN:

Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.

Sequence:
MASKVSCLYVLTVVCWASALWYLSITRPTSSYTGSKPFSHLTVARKNFTFGNIRTRPINPHSFEFLINEPNKCEKNIPFLVILISTTHKEFDARQAIRETWGDENNFKGIKIATLFLLGKNADPVLNQMVEQESQIFHDIIVEDFIDSYHNLTLKTLMGMRWVATFCSKAKYVMKTDSDIFVNMDNLIYKLLKPSTKPRRRYFTGYVINGGPIRDVRSKWYMPRDLYPDSNYPPFCSGTGYIFSADVAELIYKTSLHTRLLHLEDVYVGLCLRKLGIHPFQNSGFNHWKMAYSLCRYRRVITVHQISPEEMHRIWNDMSSKKHLRC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Chicken
80
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5Z6 As Substrate

Site PTM Type Enzyme
T114 Phosphorylation
K190 Methylation

Research Backgrounds

Function:

Beta-1,3-galactosyltransferase that transfers galactose from UDP-alpha-D-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.

Family&Domains:

Belongs to the glycosyltransferase 31 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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