DHX8 Antibody - #DF3822

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Product: DHX8 Antibody
Catalog: DF3822
Description: Rabbit polyclonal antibody to DHX8
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 150 KD; 139kD(Calculated).
Uniprot: Q14562
RRID: AB_2836179

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DHX8 Antibody detects endogenous levels of total DHX8.
RRID:
AB_2836179
Cite Format: Affinity Biosciences Cat# DF3822, RRID:AB_2836179.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase DHX8; ATP-dependent RNA helicase dhx8; DDX 8; DDX8; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8; DEAD/H box 8; DEAD/H box polypeptide 8; DEAH (Asp Glu Ala His) box polypeptide 8; DEAH box polypeptide 8; DEAH box protein 8; DHX 8; dhx8; DHX8_HUMAN; HRH 1; HRH1; PRP 22; PRP22; PRP22, S. cerevisiae, homolog of; PRPF 22; PRPF22; RNA helicase HRH 1; RNA helicase HRH1;

Immunogens

Immunogen:
Uniprot:

Q14562(DHX8_HUMAN) >>Visit HPA database.

Gene(ID):
DHX8(1659)
Sequence:
MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14562 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S129 Phosphorylation
S177 Phosphorylation
S179 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
S228 Phosphorylation
S230 Phosphorylation
Y241 Phosphorylation
T263 Phosphorylation
T273 Phosphorylation
T327 Phosphorylation
S334 Phosphorylation
T358 Phosphorylation
T362 Phosphorylation
S377 Phosphorylation
S385 Phosphorylation
S395 Phosphorylation
K399 Sumoylation
K399 Ubiquitination
S412 Phosphorylation
S456 Phosphorylation
S460 Phosphorylation
K539 Methylation
K547 Ubiquitination
T554 Phosphorylation
S557 Phosphorylation
K628 Ubiquitination
T694 Phosphorylation
K868 Ubiquitination
K885 Ubiquitination
T914 Phosphorylation
T915 Phosphorylation
S996 Phosphorylation
K1090 Ubiquitination
K1098 Ubiquitination
K1105 Ubiquitination
T1126 Phosphorylation
Y1202 Phosphorylation
Y1205 Phosphorylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

Family&Domains:

The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.

Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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