ABCD4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse liver, using ABCD4 Antibody.

Product:	ABCD4 Antibody
Catalog:	DF3814
Description:	Rabbit polyclonal antibody to ABCD4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	70 KD; 69kD(Calculated).
Uniprot:	O14678
RRID:	AB_2836171

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ABCD4 Antibody detects endogenous levels of total ABCD4.

RRID:

AB_2836171
Cite Format: Affinity Biosciences Cat# DF3814, RRID:AB_2836171.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

69 kDa peroxisomal ABC transporter; ABC 41; ABC41; ABCD 4; ABCD4; ABCD4_HUMAN; ATP binding cassette sub family D (ALD) member 4; ATP binding cassette sub family D member 4; ATP-binding cassette sub-family D member 4; EST352188; MAHCJ; MGC105956; P70R; P79R; Peroxisomal membrane protein 1 like; Peroxisomal membrane protein 1-like; Peroxisomal membrane protein 69; PMP 69; PMP69; PMP70-related protein; PXMP 1L; PXMP1 L; PXMP1-L; PXMP1L;

Immunogens

Immunogen:

Uniprot:

O14678(ABCD4_HUMAN) >>Visit HPA database.

Gene(ID):

ABCD4(5826)

Expression:

O14678 ABCD4_HUMAN:

Ubiquitous.

Sequence:

O14678 ABCD4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVAGPAPGAGARPRLDLQFLQRFLQILKVLFPSWSSQNALMFLTLLCLTLLEQFVIYQVGLIPSQYYGVLGNKDLEGFKTLTFLAVMLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHRLYFRGRAYYTLNVLRDDIDNPDQRISQDVERFCRQLSSMASKLIISPFTLVYYTYQCFQSTGWLGPVSIFGYFILGTVVNKTLMGPIVMKLVHQEKLEGDFRFKHMQIRVNAEPAAFYRAGHVEHMRTDRRLQRLLQTQRELMSKELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGDLSPAELSTLVSKNAFVCIYLISCFTQLIDLSTTLSDVAGYTHRIGQLRETLLDMSLKSQDCEILGESEWGLDTPPGWPAAEPADTAFLLERVSISAPSSDKPLIKDLSLKISEGQSLLITGNTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKEVYPDSGSADDERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEVESELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWELMRIKVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Xenopus

100

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14678 As Substrate

O14678

Site	PTM Type	Source
T81	Phosphorylation	Uniprot
T116	Phosphorylation	Uniprot
K217	Ubiquitination	Uniprot
K225	Ubiquitination	Uniprot
S398	Phosphorylation	Uniprot
S399	Phosphorylation	Uniprot
K401	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot

Research Backgrounds

O14678_ABCD4_HUMAN

Function:

May be involved in intracellular processing of vitamin B12 (cobalamin). Could play a role in the lysosomal release of vitamin B12 into the cytoplasm.

Subcellular Location:

Peroxisome membrane>Multi-pass membrane protein.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer or heterodimer.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Environmental Information Processing > Membrane transport > ABC transporters.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ABCD4 Antibody - #DF3814