ATP5I Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using ATP5I Antibody.

Product:	ATP5I Antibody
Catalog:	DF3803
Description:	Rabbit polyclonal antibody to ATP5I
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	8 KD; 8kD(Calculated).
Uniprot:	P56385
RRID:	AB_2836160

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

ATP5I Antibody detects endogenous levels of total ATP5I.

RRID:

AB_2836160
Cite Format: Affinity Biosciences Cat# DF3803, RRID:AB_2836160.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP 5I; ATP 5K; ATP synthase e chain mitochondrial; ATP synthase H+ transporting mitochondrial F0 complex subunit E; ATP synthase subunit e; ATP synthase subunit e mitochondrial; ATP5I; ATP5I_HUMAN; ATP5K; ATPase subunit e; F1F0 ATP synthase murine e subunit; MGC12532; mitochondrial;

Immunogens

Immunogen:

Uniprot:

P56385(ATP5I_HUMAN) >>Visit HPA database.

Gene(ID):

ATP5I(521)

Sequence:

P56385 ATP5I_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVPPVQVSPLIKLGRYSALFLGVAYGATRYNYLKPRAEEERRIAAEEKKKQDELKRIARELAEDDSILK

PTMs - P56385 As Substrate

P56385

Site	PTM Type	Source
S8	Phosphorylation	Uniprot
Y25	Phosphorylation	Uniprot
T28	Phosphorylation	Uniprot
Y30	Phosphorylation	Uniprot
Y32	Phosphorylation	Uniprot
K34	Acetylation	Uniprot
K34	Ubiquitination	Uniprot
K49	Ubiquitination	Uniprot
K50	Ubiquitination	Uniprot
S66	Phosphorylation	Uniprot
K69	Acetylation	Uniprot
K69	Ubiquitination	Uniprot

Research Backgrounds

P56385_ATP5I_HUMAN

Function:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

Subcellular Location:

Mitochondrion. Mitochondrion inner membrane.

Subunit Structure:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).

Family&Domains:

Belongs to the ATPase e subunit family.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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ATP5I Antibody - #DF3803