ARG1 Antibody - #DF3791

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Product: ARG1 Antibody
Catalog: DF3791
Description: Rabbit polyclonal antibody to ARG1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: P05089
RRID: AB_2836148

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(90%), Horse(89%), Sheep(90%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARG1 Antibody detects endogenous levels of total ARG1.
RRID:
AB_2836148
Cite Format: Affinity Biosciences Cat# DF3791, RRID:AB_2836148.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A I; Al; ARG 1; arg1; ARGI1_HUMAN; Arginase 1; Arginase liver; Arginase type I; Arginase, liver; Arginase-1; Arginase1; Liver type arginase; Liver-type arginase; Type I arginase;

Immunogens

Immunogen:
Uniprot:

P05089(ARGI1_HUMAN) >>Visit HPA database.

Gene(ID):
ARG1(383)
Expression:
P05089 ARGI1_HUMAN:

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) (PubMed:15546957). Also detected in macrophages mycobacterial granulomas (PubMed:23749634). Expressed in group2 innate lymphoid cells (ILC2s) during lung disease (PubMed:27043409).

Sequence:
MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
90
Sheep
90
Rabbit
90
Pig
89
Horse
89
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05089 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
T29 Phosphorylation
K33 Ubiquitination
Y50 Phosphorylation
S62 Phosphorylation
S72 Phosphorylation
S94 Phosphorylation
S102 Phosphorylation
S107 Phosphorylation
S109 Phosphorylation
S163 Phosphorylation
T166 Phosphorylation
C168 S-Nitrosylation
S170 Phosphorylation
K191 Acetylation
S217 Phosphorylation
Y265 Phosphorylation
C303 S-Nitrosylation

Research Backgrounds

Function:

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.

Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Subcellular Location:

Cytoplasm. Cytoplasmic granule.
Note: Localized in azurophil granules of neutrophils (PubMed:15546957).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Subunit Structure:

Homotrimer. Interacts with CMTM6.

Family&Domains:

Belongs to the arginase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). Metformin alleviates prolonged isoflurane inhalation induced cognitive decline via reducing neuroinflammation in adult mice. International Immunopharmacology, 2022 (PubMed: 35709590) [IF=5.6]

Application: WB    Species: Mouse    Sample: hippocampus

Fig. 3. Metformin promoted microglia cells polarization from M1 phenotype to the M2 phenotype in the hippocampus of adult mice after lengthy isoflurane exposure. Representative western blots (above panels) and densitometric analysis (below panels, n = 3 mice per group) showing the effects of lengthy isoflurane inhalation (A, D), metformin pretreatment plus isoflurane inhalation (B, E), and metformin treatment (C, F) on expression levels of M1 microglia markers (CD68, iNOS) and M2 microglia markers (IL-10, Arg-1) in the hippocampus of adult mice. Tubulin was used as an internal control. Data are expressed as mean ± SD and compared by two-tailed Student’s t test.

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