Product: AMPH Antibody
Catalog: DF3767
Description: Rabbit polyclonal antibody to AMPH
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 76 KD; 76kD(Calculated).
Uniprot: P49418
RRID: AB_2836131

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
AMPH Antibody detects endogenous levels of total AMPH.
RRID:
AB_2836131
Cite Format: Affinity Biosciences Cat# DF3767, RRID:AB_2836131.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amp; AMPH 1; AMPH; AMPH_HUMAN; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin; Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man syndrome with breast cancer 128kD autoantigen; Stiff man syndrome with breast cancer 128kDa autoantigen;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P49418 AMPH_HUMAN:

Neurons, certain endocrine cell types and spermatocytes.

Sequence:
MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAEKATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
89
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49418 As Substrate

Site PTM Type Enzyme
K5 Acetylation
K5 Ubiquitination
T6 Phosphorylation
K11 Acetylation
K15 Acetylation
K23 Acetylation
T34 Phosphorylation
S74 Phosphorylation
K141 Ubiquitination
Y145 Phosphorylation
S147 Phosphorylation
S210 Phosphorylation
S211 Phosphorylation
S250 Phosphorylation
T260 Phosphorylation
S262 Phosphorylation Q13627 (DYRK1A)
S268 Phosphorylation
S272 Phosphorylation Q00535 (CDK5) , P06493 (CDK1) , Q13627 (DYRK1A)
S276 Phosphorylation Q00535 (CDK5) , P06493 (CDK1) , Q13627 (DYRK1A)
S285 Phosphorylation P06493 (CDK1) , Q00535 (CDK5) , Q13627 (DYRK1A)
S293 Phosphorylation Q13627 (DYRK1A) , O76039 (CDKL5) , P28482 (MAPK1) , P27361 (MAPK3)
S295 Phosphorylation P28482 (MAPK1) , Q13627 (DYRK1A) , P27361 (MAPK3)
T310 Phosphorylation Q13627 (DYRK1A)
T312 Phosphorylation Q13627 (DYRK1A)
T350 Phosphorylation P68400 (CSNK2A1)
T387 Phosphorylation P68400 (CSNK2A1)
T445 Phosphorylation
T456 Phosphorylation
S506 Phosphorylation
S549 Phosphorylation
K683 Ubiquitination

Research Backgrounds

Function:

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Neurons, certain endocrine cell types and spermatocytes.

Subunit Structure:

Heterodimer with BIN1 (By similarity). Binds SH3GLB1 (By similarity). Interacts with REPS1 and SGIP1. Binds AP2A2. Interacts with AP2B1. Interacts with DNM1 AND SYNJ1 (By similarity).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

Restrictive clause

 

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