AASS Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat liver, using AASS Antibody.

Product:	AASS Antibody
Catalog:	DF3763
Description:	Rabbit polyclonal antibody to AASS
Application:	WB IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	102 KD; 102kD(Calculated).
Uniprot:	Q9UDR5
RRID:	AB_2836127

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%)

Clonality:

Polyclonal

Specificity:

AASS Antibody detects endogenous levels of total AASS.

RRID:

AB_2836127
Cite Format: Affinity Biosciences Cat# DF3763, RRID:AB_2836127.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha aminoadipic semialdehyde synthase mitochondrial; LKR; LKR/SDH; LKRSDH; LOR; LORSDH; Lysine ketoglutarate reductase; Saccharopine dehydrogenase; SDH;

Immunogens

Immunogen:

Uniprot:

Q9UDR5(AASS_HUMAN) >>Visit HPA database.

Gene(ID):

AASS(10157)

Expression:

Q9UDR5 AASS_HUMAN:

Expressed in all 16 tissues examined with highest expression in the liver.

Sequence:

Q9UDR5 AASS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLQVHRTGLGRLGVSLSKGLHHKAVLAVRREDVNAWERRAPLAPKHIKGITNLGYKVLIQPSNRRAIHDKDYVKAGGILQEDISEACLILGVKRPPEEKLMSRKTYAFFSHTIKAQEANMGLLDEILKQEIRLIDYEKMVDHRGVRVVAFGQWAGVAGMINILHGMGLRLLALGHHTPFMHIGMAHNYRNSSQAVQAVRDAGYEISLGLMPKSIGPLTFVFTGTGNVSKGAQAIFNELPCEYVEPHELKEVSQTGDLRKVYGTVLSRHHHLVRKTDAVYDPAEYDKHPERYISRFNTDIAPYTTCLINGIYWEQNTPRLLTRQDAQSLLAPGKFSPAGVEGCPALPHKLVAICDISADTGGSIEFMTECTTIEHPFCMYDADQHIIHDSVEGSGILMCSIDNLPAQLPIEATECFGDMLYPYVEEMILSDATQPLESQNFSPVVRDAVITSNGTLPDKYKYIQTLRESRERAQSLSMGTRRKVLVLGSGYISEPVLEYLSRDGNIEITVGSDMKNQIEQLGKKYNINPVSMDICKQEEKLGFLVAKQDLVISLLPYVLHPLVAKACITNKVNMVTASYITPALKELEKSVEDAGITIIGELGLDPGLDHMLAMETIDKAKEVGATIESYISYCGGLPAPEHSNNPLRYKFSWSPVGVLMNVMQSATYLLDGKVVNVAGGISFLDAVTSMDFFPGLNLEGYPNRDSTKYAEIYGISSAHTLLRGTLRYKGYMKALNGFVKLGLINREALPAFRPEANPLTWKQLLCDLVGISPSSEHDVLKEAVLKKLGGDNTQLEAAEWLGLLGDEQVPQAESILDALSKHLVMKLSYGPEEKDMIVMRDSFGIRHPSGHLEHKTIDLVAYGDINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLMGPFSKEIYGPILERIKAEGIIYTTQSTIKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UDR5 As Substrate

Q9UDR5

Site	PTM Type	Source
T51	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
K212	Acetylation	Uniprot
K229	Acetylation	Uniprot
T263	Phosphorylation	Uniprot
Y461	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
T479	Phosphorylation	Uniprot
S492	Phosphorylation	Uniprot
K539	Ubiquitination	Uniprot
Y556	Phosphorylation	Uniprot
Y700	Phosphorylation	Uniprot
K707	Acetylation	Uniprot
Y730	Phosphorylation	Uniprot
T878	Phosphorylation	Uniprot
K893	Ubiquitination	Uniprot

Research Backgrounds

Q9UDR5_AASS_HUMAN

Function:

Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively.

Subcellular Location:

Mitochondrion.

Tissue Specificity:

Expressed in all 16 tissues examined with highest expression in the liver.

Subunit Structure:

Homodimer.

Family&Domains:

In the N-terminal section; belongs to the AlaDH/PNT family.

In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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AASS Antibody - #DF3763