Product: AKR1B1 Antibody
Catalog: DF3759
Description: Rabbit polyclonal antibody to AKR1B1
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 36 KD; 36kD(Calculated).
Uniprot: P15121
RRID: AB_2836123

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AKR1B1 Antibody detects endogenous levels of total AKR1B1.
RRID:
AB_2836123
Cite Format: Affinity Biosciences Cat# DF3759, RRID:AB_2836123.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADR; AKR1B 1; Akr1b1; Aldehyde reductase 1; Aldehyde reductase; Aldo keto reductase family 1, member B1; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldose reductase; aldr 1; ALDR_HUMAN; aldr1; ALR2; AR; Lii5 2 CTCL tumor antigen; Low Km aldose reductase; MGC1804;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P15121 ALDR_HUMAN:

Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

Sequence:
MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
90
Rabbit
90
Zebrafish
80
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15121 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
K12 Acetylation
K12 Ubiquitination
T20 Phosphorylation
K22 Ubiquitination
S23 Phosphorylation
Y40 Phosphorylation
Y49 Phosphorylation
K62 Ubiquitination
K69 Ubiquitination
S77 Phosphorylation
Y83 Phosphorylation
K86 Ubiquitination
K90 Ubiquitination
K95 Acetylation
K95 Ubiquitination
S98 Phosphorylation
Y104 Phosphorylation
K117 Acetylation
K179 Ubiquitination
Y190 Phosphorylation
T192 Phosphorylation
K195 Ubiquitination
Y199 Phosphorylation
S211 Phosphorylation
S215 Phosphorylation
R218 Methylation
K222 Acetylation
K222 Methylation
K222 Ubiquitination
R233 Methylation
K240 Ubiquitination
K243 Acetylation
K243 Ubiquitination
K263 Acetylation
K263 Ubiquitination
S264 Phosphorylation
T266 Phosphorylation
C299 S-Nitrosylation
C304 S-Nitrosylation
K308 Acetylation
K308 Ubiquitination

Research Backgrounds

Function:

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the aldo/keto reductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports, 2021 (PubMed: 34490476) [IF=3.4]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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