ALDH1B1 Antibody - #DF3755

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Product: ALDH1B1 Antibody
Catalog: DF3755
Description: Rabbit polyclonal antibody to ALDH1B1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Dog
Mol.Wt.: 57 KD; 57kD(Calculated).
Uniprot: P30837
RRID: AB_2836119

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Dog(80%)
Clonality:
Polyclonal
Specificity:
ALDH1B1 Antibody detects endogenous levels of total ALDH1B1.
RRID:
AB_2836119
Cite Format: Affinity Biosciences Cat# DF3755, RRID:AB_2836119.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acetaldehyde dehydrogenase 5; AL1B1_HUMAN; Aldehyde dehydrogenase 1 family, member B1; Aldehyde dehydrogenase 1B1; Aldehyde dehydrogenase 5; Aldehyde dehydrogenase family 1 member B1; Aldehyde dehydrogenase X; Aldehyde dehydrogenase X mitochondrial; ALDH class 2; Aldh1b1; ALDH5; ALDHX; MGC2230; Mitochondrial aldehyde dehydrogenase X; mitochondrial; OTTHUMP00000021399;

Immunogens

Immunogen:
Uniprot:

P30837(AL1B1_HUMAN) >>Visit HPA database.

Gene(ID):
ALDH1B1(219)
Expression:
P30837 AL1B1_HUMAN:

Liver, testis and to a lesser extent in brain.

Sequence:
MLRFLAPRLLSLQGRTARYSSAAALPSPILNPDIPYNQLFINNEWQDAVSKKTFPTVNPTTGEVIGHVAEGDRADVDRAVKAAREAFRLGSPWRRMDASERGRLLNLLADLVERDRVYLASLETLDNGKPFQESYALDLDEVIKVYRYFAGWADKWHGKTIPMDGQHFCFTRHEPVGVCGQIIPWNFPLVMQGWKLAPALATGNTVVMKVAEQTPLSALYLASLIKEAGFPPGVVNIITGYGPTAGAAIAQHVDVDKVAFTGSTEVGHLIQKAAGDSNLKRVTLELGGKSPSIVLADADMEHAVEQCHEALFFNMGQCCCAGSRTFVEESIYNEFLERTVEKAKQRKVGNPFELDTQQGPQVDKEQFERVLGYIQLGQKEGAKLLCGGERFGERGFFIKPTVFGGVQDDMRIAKEEIFGPVQPLFKFKKIEEVVERANNTRYGLAAAVFTRDLDKAMYFTQALQAGTVWVNTYNIVTCHTPFGGFKESGNGRELGEDGLKAYTEVKTVTIKVPQKNS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Dog
80
Bovine
70
Xenopus
60
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30837 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S21 Phosphorylation
S27 Phosphorylation
K52 Ubiquitination
R73 Methylation
Y135 Phosphorylation
S217 Phosphorylation
T283 Phosphorylation
S330 Phosphorylation
Y332 Phosphorylation
K347 Ubiquitination
K364 Acetylation
K364 Ubiquitination
Y373 Phosphorylation
K379 Acetylation
K383 Ubiquitination
K399 Acetylation
T401 Phosphorylation
K426 Ubiquitination
K500 Ubiquitination

Research Backgrounds

Function:

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver, testis and to a lesser extent in brain.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the aldehyde dehydrogenase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Amino acid metabolism > Histidine metabolism.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2023 (PubMed: 36773462) [IF=6.2]

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