ARFGAP1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using ARFGAP1 Antibody.

Product:	ARFGAP1 Antibody
Catalog:	DF3747
Description:	Rabbit polyclonal antibody to ARFGAP1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit
Mol.Wt.:	60/48 KD; 45kD(Calculated).
Uniprot:	Q8N6T3
RRID:	AB_2836111

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(88%)

Clonality:

Polyclonal

Specificity:

ARFGAP1 Antibody detects endogenous levels of total ARFGAP1.

RRID:

AB_2836111
Cite Format: Affinity Biosciences Cat# DF3747, RRID:AB_2836111.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADP ribosylation factor 1 GTPase activating protein; ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF1 directed GTPase activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1_HUMAN; Arfgap1; GAP protein; HRIHFB2281;

Immunogens

Immunogen:

Uniprot:

Q8N6T3(ARFG1_HUMAN) >>Visit HPA database.

Gene(ID):

ARFGAP1(55738)

Sequence:

Q8N6T3 ARFG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Sheep

Horse

Bovine

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N6T3 As Substrate

Q8N6T3

Site	PTM Type	Source
S57	Phosphorylation	Uniprot
T63	Phosphorylation	Uniprot
K68	Ubiquitination	Uniprot
K74	Acetylation	Uniprot
K74	Ubiquitination	Uniprot
K82	Ubiquitination	Uniprot
Y93	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
K102	Ubiquitination	Uniprot
Y103	Phosphorylation	Uniprot
S105	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
S125	Phosphorylation	Uniprot
S128	Phosphorylation	Uniprot
S129	Phosphorylation	Uniprot
T135	Phosphorylation	Uniprot
T141	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S155	Phosphorylation	Uniprot
T157	Phosphorylation	Uniprot
S160	Phosphorylation	Uniprot
K162	Ubiquitination	Uniprot
S174	Phosphorylation	Uniprot
Y175	Phosphorylation	Uniprot
T189	Phosphorylation	Uniprot
K194	Ubiquitination	Uniprot
S206	Phosphorylation	Uniprot
Y208	Phosphorylation	Uniprot
S209	Phosphorylation	Uniprot
K226	Acetylation	Uniprot
K226	Ubiquitination	Uniprot
K231	Acetylation	Uniprot
K231	Sumoylation	Uniprot
K231	Ubiquitination	Uniprot
S237	Phosphorylation	Uniprot
K239	Ubiquitination	Uniprot
S241	Phosphorylation	Uniprot
S246	Phosphorylation	Uniprot
K253	Acetylation	Uniprot
K253	Ubiquitination	Uniprot
K263	Acetylation	Uniprot
K263	Ubiquitination	Uniprot
S273	Phosphorylation	Uniprot
K278	Ubiquitination	Uniprot
S284	Phosphorylation	Uniprot
K285	Ubiquitination	Uniprot
S295	Phosphorylation	Uniprot
K297	Sumoylation	Uniprot
S304	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
S310	Phosphorylation	Uniprot
Y311	Phosphorylation	Uniprot
S334	Phosphorylation	Uniprot
K339	Ubiquitination	Uniprot
K342	Ubiquitination	Uniprot
S343	Phosphorylation	Uniprot
S345	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
T350	Phosphorylation	Uniprot
C351	S-Nitrosylation	Uniprot
S360	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
S363	Phosphorylation	Uniprot
S376	Phosphorylation	Uniprot
S378	Phosphorylation	Uniprot

Research Backgrounds

Q8N6T3_ARFG1_HUMAN

Function:

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

Subcellular Location:

Cytoplasm. Golgi apparatus.
Note: Associates with the Golgi complex.

Subunit Structure:

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. The interaction with TMED2 inhibits the GAP activity (By similarity).

Family&Domains:

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ARFGAP1 Antibody - #DF3747