ARF4 Antibody - #DF3745

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Product: ARF4 Antibody
Catalog: DF3745
Description: Rabbit polyclonal antibody to ARF4
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 KD; 21kD(Calculated).
Uniprot: P18085
RRID: AB_2836109

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(80%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
ARF4 Antibody detects endogenous levels of total ARF4.
RRID:
AB_2836109
Cite Format: Affinity Biosciences Cat# DF3745, RRID:AB_2836109.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor 2; ADP ribosylation factor 4; ADP-ribosylation factor 4; ARF2; ARF4; ARF4_HUMAN;

Immunogens

Immunogen:
Uniprot:

P18085(ARF4_HUMAN) >>Visit HPA database.

Gene(ID):
ARF4(378)
Sequence:
MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Rabbit
90
Chicken
80
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18085 As Substrate

Site PTM Type Enzyme
M1 Acetylation
G2 Myristoylation
K15 Ubiquitination
K16 Ubiquitination
Y35 Phosphorylation
K36 Acetylation
K36 Ubiquitination
K38 Ubiquitination
Y58 Phosphorylation
K109 Ubiquitination
K127 Ubiquitination
S137 Phosphorylation
K142 Ubiquitination
S147 Phosphorylation
T152 Phosphorylation
Y167 Phosphorylation

Research Backgrounds

Function:

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Subcellular Location:

Golgi apparatus. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the small GTPase superfamily. Arf family.

References

1). Bisimidazolium Salt Glycosyltransferase Inhibitors Suppress Hepatocellular Carcinoma Progression In Vitro and In Vivo. Pharmaceuticals, 2022 (PubMed: 35745636) [IF=4.6]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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