BST1 Antibody - #DF3744

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Product: BST1 Antibody
Catalog: DF3744
Description: Rabbit polyclonal antibody to BST1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog, Chicken
Mol.Wt.: 34 KD; 36kD(Calculated).
Uniprot: Q10588
RRID: AB_2836108

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Rabbit(80%), Dog(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
BST1 Antibody detects endogenous levels of total BST1.
RRID:
AB_2836108
Cite Format: Affinity Biosciences Cat# DF3744, RRID:AB_2836108.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosyl cyclase 2; ADP-ribosyl cyclase 2; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; Bone marrow stromal antigen 1; Bone marrow stromal cell antigen 1; BST 1; BST-1; BST1; BST1_HUMAN; cADPr hydrolase 2; CD157; CD157 antigen; Cyclic ADP ribose hydrolase 2; Cyclic ADP-ribose hydrolase 2; NAD(+) nucleosidase; OTTHUMP00000217617;

Immunogens

Immunogen:
Uniprot:

Q10588(BST1_HUMAN) >>Visit HPA database.

Gene(ID):
BST1(683)
Expression:
Q10588 BST1_HUMAN:

Widely expressed.

Sequence:
MAAQGCAASRLLQLLLQLLLLLLLLAAGGARARWRGEGTSAHLRDIFLGRCAEYRALLSPEQRNKNCTAIWEAFKVALDKDPCSVLPSDYDLFINLSRHSIPRDKSLFWENSHLLVNSFADNTRRFMPLSDVLYGRVADFLSWCRQKNDSGLDYQSCPTSEDCENNPVDSFWKRASIQYSKDSSGVIHVMLNGSEPTGAYPIKGFFADYEIPNLQKEKITRIEIWVMHEIGGPNVESCGEGSMKVLEKRLKDMGFQYSCINDYRPVKLLQCVDHSTHPDCALKSAAAATQRKAPSLYTEQRAGLIIPLFLVLASRTQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
90
Chicken
80
Rabbit
80
Xenopus
70
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q10588 As Substrate

Site PTM Type Enzyme
N95 N-Glycosylation
N192 N-Glycosylation
Y263 Phosphorylation

Research Backgrounds

Function:

Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth.

Subcellular Location:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the ADP-ribosyl cyclase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast. Redox Biology (PubMed: 35926314) [IF=11.4]

Application: WB    Species: Mouse    Sample:

Fig. 5. NAD + depletion in pregnant mice triggered by DEHP. Pregnant mice were administrated by DEHP (0, 5, 50 and 200 mg/kg/d) during GD 0–15. HTR-8 cells were treated with MEHP (0, 20, 200, 500 μM) for 24 h. NAD+ content was measured in (A–D) serum, (E–H) placenta, and (I–L) HTR-8 cells, respectively. (M) CD38, CD157, and (N) NAMPT representative images of Western blot. Quantifications of (O) CD38, (P) CD157, and (Q) NAMPT expression in mice placenta based on band intensity were shown below the blots. All data are expressed as mean ± standard errors. (N = 6). * indicate a statistically significant difference compared to control (P < 0.05).

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