SLC25A6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293, using SLC25A6 Antibody.

DF3742 at 1/100 staining Mouse kidney tissue by IHC-P.

DF3742 at 1/100 staining Rat kidney tissue by IHC-P.

DF3742 at 1/100 staining Rat ovarian tissue by IHC-P.

Product:	SLC25A6 Antibody
Catalog:	DF3742
Description:	Rabbit polyclonal antibody to SLC25A6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Dog
Mol.Wt.:	32 KD; 33kD(Calculated).
Uniprot:	P12236
RRID:	AB_2836106

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(90%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SLC25A6 Antibody detects endogenous levels of total SLC25A6.

RRID:

AB_2836106
Cite Format: Affinity Biosciences Cat# DF3742, RRID:AB_2836106.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AAC3; Adenine nucleotide translocator 3; ADP; ADP ATP carrier protein 3; ADP ATP carrier protein isoform T2; ADP ATP carrier protein liver; ADP/ATP translocase 3; ADP/ATP translocator of liver; ADT3_HUMAN; ANT 2; ANT 3; ANT; ANT3; ANT3Y; ATP carrier protein 3; ATP carrier protein; CDABP0051; isoform T2; MGC17525; SLC25A6; Solute carrier family 25 (mitochondrial carrier adenine nucleotide translocator) member 6; Solute carrier family 25 member 6;

Immunogens

Immunogen:

Uniprot:

P12236(ADT3_HUMAN) >>Visit HPA database.

Gene(ID):

SLC25A6(293)

Sequence:

P12236 ADT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTEQAISFAKDFLAGGIAAAISKTAVAPIERVKLLLQVQHASKQIAADKQYKGIVDCIVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKHTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKSGTEREFRGLGDCLVKITKSDGIRGLYQGFSVSVQGIIIYRAAYFGVYDTAKGMLPDPKNTHIVVSWMIAQTVTAVAGVVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIFRDEGGKAFFKGAWSNVLRGMGGAFVLVLYDELKKVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Bovine

Zebrafish

Chicken

Horse

Sheep

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12236 As Substrate

P12236

Site	PTM Type	Source
M1	Acetylation	Uniprot
T2	Acetylation	Uniprot
K10	Acetylation	Uniprot
K10	Methylation	Uniprot
K10	Ubiquitination	Uniprot
S22	Phosphorylation	Uniprot
K23	Acetylation	Uniprot
K23	Ubiquitination	Uniprot
T24	Phosphorylation	Uniprot
K33	Acetylation	Uniprot
K33	Ubiquitination	Uniprot
S42	Phosphorylation	Uniprot
K43	Acetylation	Uniprot
K43	Ubiquitination	Uniprot
K49	Methylation	Uniprot
K49	Ubiquitination	Uniprot
Y51	Phosphorylation	Uniprot
K52	Methylation	Uniprot
K63	Acetylation	Uniprot
K63	Ubiquitination	Uniprot
S69	Phosphorylation	Uniprot
Y81	Phosphorylation	Uniprot
T84	Phosphorylation	Uniprot
K92	Acetylation	Uniprot
K92	Ubiquitination	Uniprot
K94	Acetylation	Uniprot
K96	Acetylation	Uniprot
K96	Ubiquitination	Uniprot
K105	Acetylation	Uniprot
K105	Ubiquitination	Uniprot
Y112	Phosphorylation	Uniprot
S119	Phosphorylation	Uniprot
T139	Phosphorylation	Uniprot
R140	Methylation	Uniprot
K147	Acetylation	Uniprot
K147	Methylation	Uniprot
K147	Ubiquitination	Uniprot
S148	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
R155	Methylation	Uniprot
C160	S-Nitrosylation	Uniprot
K163	Acetylation	Uniprot
K163	Ubiquitination	Uniprot
K166	Ubiquitination	Uniprot
Y191	Phosphorylation	Uniprot
Y195	Phosphorylation	Uniprot
T197	Phosphorylation	Uniprot
K199	Sumoylation	Uniprot
K199	Ubiquitination	Uniprot
K206	Sumoylation	Uniprot
Y229	Phosphorylation	Uniprot
S242	Phosphorylation	Uniprot
K245	Ubiquitination	Uniprot
K260	Ubiquitination	Uniprot
K268	Acetylation	Uniprot
K268	Ubiquitination	Uniprot
K272	Acetylation	Uniprot
K272	Methylation	Uniprot
K272	Ubiquitination	Uniprot
S276	Phosphorylation	Uniprot

Research Backgrounds

P12236_ADT3_HUMAN

Function:

Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

PTMs:

Trimethylated by ANTKMT at Lys-52.

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.
Note: The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria.

Subunit Structure:

Found in a complex with ARL2, ARL2BP and SLC25A6 (By similarity). Homodimer.

(Microbial infection) Interacts with influenza A virus PB1-F2 protein.

(Microbial infection) Interacts with HIV-1 Vpr.

Family&Domains:

The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue.

Belongs to the mitochondrial carrier (TC 2.A.29) family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SLC25A6 Antibody - #DF3742