Product: LYPLA1 Antibody
Catalog: DF3737
Description: Rabbit polyclonal antibody to LYPLA1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 KD; 25kD(Calculated).
Uniprot: O75608
RRID: AB_2836101

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
LYPLA1 Antibody detects endogenous levels of total LYPLA1.
RRID:
AB_2836101
Cite Format: Affinity Biosciences Cat# DF3737, RRID:AB_2836101.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acyl protein thioesterase 1; Acyl-protein thioesterase 1; APT 1; APT-1; APT1; hAPT1; LPL-I; LPL1; LYPA1_HUMAN; LYPLA 1; LYPLA1; Lysophospholipase 1; Lysophospholipase I; Lysophospholipid specific lysophospholipase; LYSOPLA; LysoPLA I;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Dog
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75608 As Substrate

Site PTM Type Enzyme
K97 Ubiquitination
K105 Ubiquitination
K190 Ubiquitination
S209 Phosphorylation
S210 Phosphorylation
C211 S-Nitrosylation
K224 Ubiquitination

Research Backgrounds

Function:

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.

Research Fields

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

References

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience, 2022 (PubMed: 36419851) [IF=5.8]

Application: WB    Species: Mouse    Sample: renal medulla

Figure 1 E. faecalis treatment increased blood pressure and renal LYPLA1 expression in mice (A) Systolic blood pressure (SBP, n = 5 per group) and diastolic blood pressure (DBP, n = 5 per group). (B) Serum Ang II levels (n = 5 per group). (C) Representative immunoblots of LYPLA1 and summarized intensities of blots in the renal medulla (n = 5 per group). (D) mRNA levels of LYPLA1 in the renal medulla (n = 5 per group). (E) Urinary LYPLA1 content (n = 5 per group). (F) Representative immunochemistry staining of LYPLA1 in the kidney and summarized intensities of positive staining (n = 5 per group, bar = 100 μm). Data are presented as mean ± SE. ∗p < 0.05, ∗∗p < 0.01; ns, no significance.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.