LYPLA1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using LYPLA1 Antibody.

Product:	LYPLA1 Antibody
Catalog:	DF3737
Description:	Rabbit polyclonal antibody to LYPLA1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	25 KD; 25kD(Calculated).
Uniprot:	O75608
RRID:	AB_2836101

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Zebrafish(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

LYPLA1 Antibody detects endogenous levels of total LYPLA1.

RRID:

AB_2836101
Cite Format: Affinity Biosciences Cat# DF3737, RRID:AB_2836101.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acyl protein thioesterase 1; Acyl-protein thioesterase 1; APT 1; APT-1; APT1; hAPT1; LPL-I; LPL1; LYPA1_HUMAN; LYPLA 1; LYPLA1; Lysophospholipase 1; Lysophospholipase I; Lysophospholipid specific lysophospholipase; LYSOPLA; LysoPLA I;

Immunogens

Immunogen:

Uniprot:

O75608(LYPA1_HUMAN) >>Visit HPA database.

Gene(ID):

LYPLA1(10434)

Sequence:

O75608 LYPA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Pig

Horse

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75608 As Substrate

O75608

Site	PTM Type	Source
K97	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
K190	Ubiquitination	Uniprot
S209	Phosphorylation	Uniprot
S210	Phosphorylation	Uniprot
C211	S-Nitrosylation	Uniprot
K224	Ubiquitination	Uniprot

Research Backgrounds

O75608_LYPA1_HUMAN

Function:

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.

Research Fields

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

References

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience (PubMed: 36419851) [IF=5.8]

Application: WB Species: Mouse Sample: renal medulla

Figure 1 E. faecalis treatment increased blood pressure and renal LYPLA1 expression in mice (A) Systolic blood pressure (SBP, n = 5 per group) and diastolic blood pressure (DBP, n = 5 per group). (B) Serum Ang II levels (n = 5 per group). (C) Representative immunoblots of LYPLA1 and summarized intensities of blots in the renal medulla (n = 5 per group). (D) mRNA levels of LYPLA1 in the renal medulla (n = 5 per group). (E) Urinary LYPLA1 content (n = 5 per group). (F) Representative immunochemistry staining of LYPLA1 in the kidney and summarized intensities of positive staining (n = 5 per group, bar = 100 μm). Data are presented as mean ± SE. ∗p < 0.05, ∗∗p < 0.01; ns, no significance.

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LYPLA1 Antibody - #DF3737