ACOT2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells using ACOT2 antibody.

Product:	ACOT2 Antibody
Catalog:	DF3734
Description:	Rabbit polyclonal antibody to ACOT2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine
Mol.Wt.:	53 KD; 53kD(Calculated).
Uniprot:	P49753
RRID:	AB_2836098

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(90%)

Clonality:

Polyclonal

Specificity:

ACOT2 Antibody detects endogenous levels of total ACOT2.

RRID:

AB_2836098
Cite Format: Affinity Biosciences Cat# DF3734, RRID:AB_2836098.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AA571646; ACOT2; Acyl CoA thioesterase 2; Acyl coenzyme A thioester hydrolase 2a; Acyl coenzyme A thioesterase 2, mitochondrial; CTE Ia; CTE1A; Long chain acyl CoA thioesterase 2; Mitochondrial acyl CoA thioesterase 1; Mitochondrial acyl CoA thioesterase 2; MTE1; Peroxisomal long chain acyl coA; PTE2; PTE2A; ZAP128;

Immunogens

Immunogen:

Uniprot:

P49753(ACOT2_HUMAN) >>Visit HPA database.

Gene(ID):

ACOT2(10965)

Expression:

P49753 ACOT2_HUMAN:

Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.

Sequence:

P49753 ACOT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSNKLLSPHPHSVVLRSEFKMASSPAVLRASRLYQWSLKSSAQFLGSPQLRQVGQIIRVPARMAATLILEPAGRCCWDEPVRIAVRGLAPEQPVTLRASLRDEKGALFQAHARYRADTLGELDLERAPALGGSFAGLEPMGLLWALEPEKPLVRLVKRDVRTPLAVELEVLDGHDPDPGRLLCQTRHERYFLPPGVRREPVRVGRVRGTLFLPPEPGPFPGIVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLHYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHAMAQVDAWKQLQTFFHKHLGGHEGTIPSKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Xenopus

Pig

Horse

Sheep

Dog

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49753 As Substrate

P49753

Site	PTM Type	Source
S7	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
S47	Phosphorylation	Uniprot
T66	Phosphorylation	Uniprot
R86	Methylation	Uniprot
K104	Acetylation	Uniprot
T118	Phosphorylation	Uniprot
T162	Phosphorylation	Uniprot
S294	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
Y351	Phosphorylation	Uniprot
K368	Ubiquitination	Uniprot
K402	Acetylation	Uniprot
K462	Acetylation	Uniprot
K482	Ubiquitination	Uniprot

Research Backgrounds

P49753_ACOT2_HUMAN

Function:

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 2/ACOT2 displays higher activity toward long chain acyl CoAs (C14-C20). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria (By similarity).

Subcellular Location:

Mitochondrion.

Tissue Specificity:

Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the C/M/P thioester hydrolase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

Restrictive clause

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For Research Use Only.
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ACOT2 Antibody - #DF3734