ACOT1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from Jurkat cells, using ACOT1 antibody.

DF3733 at 1/100 staining mouse liver tissue by IHC-P.

Product:	ACOT1 Antibody
Catalog:	DF3733
Description:	Rabbit polyclonal antibody to ACOT1
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	46 KD; 46kD(Calculated).
Uniprot:	Q86TX2
RRID:	AB_2836097

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

ACOT1 Antibody detects endogenous levels of total ACOT1.

RRID:

AB_2836097
Cite Format: Affinity Biosciences Cat# DF3733, RRID:AB_2836097.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACH2; Acyl CoA thioesterase 1, cytosolic; Acyl coenzyme A thioesterase 1; CTE 1; CTE I; CTE Ib; Cte1; Cytosolic acyl CoA thioesterase 1; D12Ucla1; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl CoA hydrolase; Long chain acyl CoA thioester hydrolase;

Immunogens

Immunogen:

Uniprot:

Q86TX2(ACOT1_HUMAN) >>Visit HPA database.

Gene(ID):

ACOT1(641371)

Sequence:

Q86TX2 ACOT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAATLILEPAGRCCWDEPVRIAVRGLAPEQPVTLRASLRDEKGALFQAHARYRADTLGELDLERAPALGGSFAGLEPMGLLWALEPEKPLVRLVKRDVRTPLAVELEVLDGHDPDPGRLLCRVRHERYFLPPGVRREPVRAGRVRGTLFLPPEPGPFPGIVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAVNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHAMAQVDAWKQLQTFFHKHLGGHEGTIPSKV

PTMs - Q86TX2 As Substrate

Q86TX2

Site	PTM Type	Source
R24	Methylation	Uniprot
K42	Acetylation	Uniprot
T56	Phosphorylation	Uniprot
T100	Phosphorylation	Uniprot
S232	Phosphorylation	Uniprot
S257	Phosphorylation	Uniprot
K286	Ubiquitination	Uniprot
Y289	Phosphorylation	Uniprot
K306	Ubiquitination	Uniprot
K340	Acetylation	Uniprot
K400	Acetylation	Uniprot
K420	Ubiquitination	Uniprot

Research Backgrounds

Q86TX2_ACOT1_HUMAN

Function:

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the C/M/P thioester hydrolase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ACOT1 Antibody - #DF3733