ANP32B Antibody - #DF3730

(2)  
Product: ANP32B Antibody
Catalog: DF3730
Description: Rabbit polyclonal antibody to ANP32B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.: 29 KD; 29kD(Calculated).
Uniprot: Q92688
RRID: AB_2836094

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ANP32B Antibody detects endogenous levels of total ANP32B.
RRID:
AB_2836094
Cite Format: Affinity Biosciences Cat# DF3730, RRID:AB_2836094.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acidic (leucine rich) nuclear phosphoprotein 32 family member B; Acidic leucine rich nuclear phosphoprotein 32 family member B; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; AN32B_HUMAN; ANP 32B; ANP32B; APRIL; OTTHUMP0000006377; PAL 31; PAL31; PHAPI 2; PHAPI2; PHAPI2 protein; PHAPI2a; Proliferation related acidic leucine rich protein; Putative HLA-DR-associated protein I-2; Silver stainable protein SSP 29; Silver stainable protein SSP29; Silver-stainable protein SSP29; Ssp 29; Ssp29;

Immunogens

Immunogen:
Uniprot:

Q92688(AN32B_HUMAN) >>Visit HPA database.

Gene(ID):
ANP32B(10541)
Expression:
Q92688 AN32B_HUMAN:

Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.

Sequence:
MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
83
Xenopus
50
Chicken
50
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92688 As Substrate

Site PTM Type Enzyme
R12 Methylation
T15 Phosphorylation
C27 S-Nitrosylation
K33 Acetylation
K67 Ubiquitination
K68 Sumoylation
K68 Ubiquitination
R75 Methylation
K86 Acetylation
K86 Ubiquitination
S96 Phosphorylation
K99 Acetylation
K99 Ubiquitination
K101 Acetylation
K101 Ubiquitination
S104 Phosphorylation
K110 Acetylation
K116 Acetylation
K116 Ubiquitination
S117 Phosphorylation
T126 Phosphorylation
K137 Ubiquitination
Y148 Phosphorylation
S158 Phosphorylation
K236 Acetylation
K239 Acetylation
T244 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.

PTMs:

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

Subcellular Location:

Nucleus.
Note: Accumulates in the nuclei at the S phase.

Cytoplasm.
Note: Lacks a nuclear localization signal.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.

Subunit Structure:

Monomer. Interacts with histones H3 and H4.

Family&Domains:

Histone binding is mediated by the concave surface of the LRR region.

Belongs to the ANP32 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.