ACSA Antibody - #DF3728
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3728, RRID:AB_2836092.
Fold/Unfold
ACAS2; AceCS; Acetate CoA ligase; Acetate thiokinase; Acetate--CoA ligase; Acetyl CoA synthetase; Acetyl Coenzyme A synthetase 2 (ADP forming); Acetyl coenzyme A synthetase cytoplasmic; Acetyl-CoA synthetase; Acetyl-coenzyme A synthetase; ACS; ACSA; ACSA_HUMAN; ACSS2; Acyl activating enzyme; Acyl CoA synthetase short chain family member 2; Acyl-activating enzyme; Acyl-CoA synthetase short-chain family member 2; Cytoplasmic acetyl coenzyme A synthetase; cytoplasmic; MYH7B; AceCS1
Immunogens
- Q9NR19 ACSA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLPEERVRSGSGSRGQEEAGAGGRARSWSPPPEVSRSAHVPSLQRYRELHRRSVEEPREFWGDIAKEFYWKTPCPGPFLRYNFDVTKGKIFIEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTQITYHQLLVQVCQFSNVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCSLLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQISWNQGIDLWWHELMQEAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTVGGYMLYVATTFKYVFDFHAEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NR19 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| T411 | Phosphorylation | Uniprot | |
| K418 | Acetylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| Y561 | Phosphorylation | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity).
Reversibly acetylated at Lys-661 (By similarity). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3 (By similarity).
Cytoplasm>Cytosol.
Monomer.
Belongs to the ATP-dependent AMP-binding enzyme family.
Research Fields
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
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