RPL7 Antibody - #DF3713

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Product: RPL7 Antibody
Catalog: DF3713
Description: Rabbit polyclonal antibody to RPL7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32 KD; 29kD(Calculated).
Uniprot: P18124
RRID: AB_2836077

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RPL7 Antibody detects endogenous levels of total RPL7.
RRID:
AB_2836077
Cite Format: Affinity Biosciences Cat# DF3713, RRID:AB_2836077.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L7; humL7 1; L7; Ribosomal protein L7; RL7_HUMAN; RPL 7; RPL7; Rpl7a; Surf 3;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL7, corresponding to a region within C-terminal amino acids.

Uniprot:

P18124(RL7_HUMAN) >>Visit HPA database.

Gene(ID):
RPL7(6129)
Sequence:
MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18124 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K8 Ubiquitination
T17 Phosphorylation
K19 Ubiquitination
K20 Ubiquitination
K29 Ubiquitination
K36 Ubiquitination
K40 Acetylation
K40 Ubiquitination
K48 Ubiquitination
K53 Ubiquitination
K59 Ubiquitination
K77 Ubiquitination
K88 Ubiquitination
R96 Methylation
K104 Ubiquitination
K107 Ubiquitination
K124 Acetylation
K124 Methylation
K124 Ubiquitination
K127 Ubiquitination
S129 Phosphorylation
R134 Methylation
Y139 Phosphorylation
K148 Methylation
K148 Ubiquitination
S149 Phosphorylation
Y155 Phosphorylation
K156 Methylation
K156 Ubiquitination
Y159 Phosphorylation
K161 Acetylation
T170 Phosphorylation
K181 Ubiquitination
Y195 Phosphorylation
T196 Phosphorylation
K199 Ubiquitination
K202 Methylation
K202 Ubiquitination
K212 Ubiquitination
S214 Phosphorylation
S215 Phosphorylation
R217 Methylation
K223 Ubiquitination

Research Backgrounds

Function:

Component of the large ribosomal subunit. Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs.

Subunit Structure:

Component of the large ribosomal subunit (Probable). Homodimer. Interacts with DHX33.

Family&Domains:

Belongs to the universal ribosomal protein uL30 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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