Product: RPL39 Antibody
Catalog: DF3711
Description: Rabbit polyclonal antibody to RPL39
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 6 KD; 6kD(Calculated).
Uniprot: P62891
RRID: AB_2836075

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL39 Antibody detects endogenous levels of total RPL39.
RRID:
AB_2836075
Cite Format: Affinity Biosciences Cat# DF3711, RRID:AB_2836075.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810465O16Rik; 60S ribosomal protein L39; OTTHUMP00000023908; OTTMUSP00000018582; Ribosomal protein L39; RP23-43O20.1;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL39, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
78
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62891 As Substrate

Site PTM Type Enzyme
K18 Ubiquitination

Research Backgrounds

Subunit Structure:

Interacts with IMPACT.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL39 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

References

1). Deletion of ribosomal paralogs Rpl39 and Rpl39l compromises cell proliferation via protein synthesis and mitochondrial activity. The international journal of biochemistry & cell biology, 2021 (PubMed: 34428590) [IF=4.0]

Application: WB    Species: Mouse    Sample: NIH3T3 cells

Fig. 1. Expression of Rpl39 and Rpl39l paralogs in NIH3T3 cells. (A) RT-PCR showing that both Rpl39 and Rpl39l are expressed in NIH3T3 cells. Gapdh was used as the internal control. (B) Standard curves of transcript levels of Rpl39 and Rpl39l. Series dilutions of known quantities of Rpl39 and Rpl39l cDNAs were used in quantitative RT-PCR with fluorescently tagged gene specific primers. CT values were plotted against calculated copy numbers of Rpl39 and Rpl39l transcripts. (C) Determination of copy numbers of Rpl39 and Rpl39l transcripts in NIH3T3 cells. Total RNA extracted from NIH3T3 cells were used in quantitative RT-PCR with fluorescently tagged gene specific primers. Copy numbers of transcripts were calculated according to the stand curve shown in (B). Shown are averages of four independent experiments. 100 ng of total RNA was used in each experiment. (D) Gene targeting of Rpl39 and Rpl39l in NIH3T3 cells using CRISPR/Cas9 method. Schematic drawings show genomic organizations of the two genes. sgRNA targeted sequences are underlined. The resulting deletion mutations are shown below the wild type sequences, with the sequencing data on the right. The double mutations of Rpl39 and Rpl39l is generated in the Rpl39 -/- cells. Positions of gene-specific primers for genotyping are indicated by red arrows. Red arrowheads show the positions of the single nucleotide deletion. (E) Amino acid sequences of RPL39 and RPL39L. Both proteins are 51-aa long, with only three amino acids are different (blue letters). The mutants generated from gene targeting would result in aberrant peptides or early stop, as shown in red. The peptide used by Affinity for generating the antibody against RPL39 is indicated by black line. (F) Western blotting of NIH3T3 cells. Cell lysates from wild type and mutant cells were subjected to SDS-PAGE and immuno-blotted with Affinity anti-RPL39 antibody. An increase of RPL39 in Rpl39l -/- cells can be seen.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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