RPL3 Antibody - #DF3706

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Product: RPL3 Antibody
Catalog: DF3706
Description: Rabbit polyclonal antibody to RPL3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46 KD; 46kD(Calculated).
Uniprot: P39023
RRID: AB_2836070

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL3 Antibody detects endogenous levels of total RPL3.
RRID:
AB_2836070
Cite Format: Affinity Biosciences Cat# DF3706, RRID:AB_2836070.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B; HIV1 TAR RNA binding protein B; MGC103388; MGC104284; MGC72934; Ribosomal protein L3; Ribosomal protein L3, isoform a; RL3_HUMAN; rpl3; TARBP B; TARBP-B;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL3, corresponding to a region within C-terminal amino acids.

Uniprot:

P39023(RL3_HUMAN) >>Visit HPA database.

Gene(ID):
RPL3(6122)
Sequence:
MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39023 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S13 Phosphorylation
R19 Methylation
K39 Ubiquitination
T54 Phosphorylation
S65 Phosphorylation
K66 Ubiquitination
K103 Acetylation
C114 S-Nitrosylation
K115 Acetylation
K115 Ubiquitination
K120 Ubiquitination
S125 Phosphorylation
K132 Ubiquitination
K136 Ubiquitination
K148 Acetylation
K148 Ubiquitination
K155 Ubiquitination
R169 Methylation
T189 Phosphorylation
K193 Ubiquitination
K224 Ubiquitination
K229 Ubiquitination
K250 Methylation
K250 Sumoylation
K250 Ubiquitination
S265 Phosphorylation
R268 Methylation
K286 Sumoylation
K286 Ubiquitination
Y291 Phosphorylation
K294 Acetylation
K294 Sumoylation
K294 Ubiquitination
K297 Ubiquitination
K300 Sumoylation
K300 Ubiquitination
S304 Phosphorylation
T305 Phosphorylation
Y307 Phosphorylation
S310 Phosphorylation
K312 Sumoylation
K312 Ubiquitination
K341 Acetylation
K341 Ubiquitination
K342 Acetylation
K349 Ubiquitination
S350 Phosphorylation
K356 Ubiquitination
K362 Acetylation
K362 Ubiquitination
K366 Acetylation
K366 Sumoylation
K366 Ubiquitination
K373 Acetylation
K373 Methylation
K373 Sumoylation
K373 Ubiquitination
T381 Phosphorylation
K385 Ubiquitination
K386 Sumoylation
K386 Ubiquitination
K393 Acetylation
K393 Sumoylation
K393 Ubiquitination
K394 Methylation
K399 Methylation
K399 Sumoylation

Research Backgrounds

Function:

The L3 protein is a component of the large subunit of cytoplasmic ribosomes.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the large ribosomal subunit (Probable). Interacts with METTL18. Interacts with DHX33.

Family&Domains:

Belongs to the universal ribosomal protein uL3 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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