Product: RPL27A Antibody
Catalog: DF3704
Description: Rabbit polyclonal antibody to RPL27A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 20 KD; 17kD(Calculated).
Uniprot: P46776
RRID: AB_2836068

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RPL27A Antibody detects endogenous levels of total RPL27A.
RRID:
AB_2836068
Cite Format: Affinity Biosciences Cat# DF3704, RRID:AB_2836068.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L27a; FLJ43464; ribosomal protein L27a; RL27A_HUMAN; rpl27a;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL27A, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Pig
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46776 As Substrate

Site PTM Type Enzyme
R12 Methylation
S16 Phosphorylation
K47 Acetylation
K47 Ubiquitination
Y48 Phosphorylation
Y52 Phosphorylation
K55 Acetylation
K55 Methylation
K55 Ubiquitination
K59 Sumoylation
K59 Ubiquitination
R65 Methylation
S68 Phosphorylation
C70 S-Nitrosylation
K77 Ubiquitination
K92 Ubiquitination
K94 Ubiquitination
T95 Phosphorylation
K110 Acetylation
K110 Ubiquitination
K125 Ubiquitination
K138 Ubiquitination
C144 S-Nitrosylation

Research Backgrounds

PTMs:

Hydroxylated on His-39 by MINA.

Family&Domains:

Belongs to the universal ribosomal protein uL15 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.