RPL18 Antibody - #DF3700

Figure 5. RP deficiency leads to divergent cell fates after cell cycle arrest. (A) Percentages of cells within different cell cycle stages (G1, S, and G2/M) by flow cytometry experiments on A549 cells at 24 h after knockdown of indicated RPs. Three replicates were used in t-tests. (**), P < 0.01; (***), P < 0.001. (B) Changes of cell viability by MTT assays on A549 cells at 24 h after knockdown of indicated RPs. Three replicates were used in t-tests. (**), P < 0.01; (***), P < 0.001. (C) Representative of western blotting assays (left panel) and quantification of p53 (middle panel) or p21 (right panel) protein levels at 24 h after knockdown of indicated RPs (n = 2 for p53 or 3 for p21 tests). T-tests were used. (*), P < 0.05; (**), P < 0.01; (***), P < 0.001. (D) Bar plots (left panel) showing the percentage of TUNEL+ cells at 72 h after knockdown of eS8/RPS8. Representative of TUNEL staining assays (right panel) for testing apoptosis in situ in A549 cells at 72 h after knockdown of eS8/RPS8. Three replicates were used in t-test. (*), P < 0.05. (E) Bar plots (left panel) showing the percentages of β-gal-positive cells at 24, 48 and 72 h after knockdown of eL13/RPL13 or eL18/RPL18. Representative of β-gal staining assays (right panel) for testing senescence in A549 cells at 24, 48 and 72 h after knockdown of eL13/RPL13or eL18/RPL18. Three replicates were used in t-tests. (**), P < 0.01.

Figure 8. rSS1GFP infection affects the expression of cellular translation, posttranslational modification and trafficking-associated proteins. (A) The heatmap of representative 20 DEPs related to “Translation, ribosomal structure and biogenesis” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Translation, ribosomal structure and biogenesis” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “Posttranslational modification, protein turnover, chaperones” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “Posttranslational modification, protein turnover, chaperones” are analyzed by the STRING software. (E) The heatmap of representative 20 DEPs related to “Intracellular trafficking, secretion, and vesicular transport” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (F) The protein-protein interactions of the DEPs related to “Intracellular trafficking, secretion, and vesicular transport” are analyzed by the STRING software. (G) The mRNA expression levels of six selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (H) The protein expression levels of six DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of six DEPs were compared with the control GAPDH expression.