Product: RPL15 Antibody
Catalog: DF3698
Description: Rabbit polyclonal antibody to RPL15
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 20 KD; 24kD(Calculated).
Uniprot: P61313
RRID: AB_2836062

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL15 Antibody detects endogenous levels of total RPL15.
RRID:
AB_2836062
Cite Format: Affinity Biosciences Cat# DF3698, RRID:AB_2836062.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L15; EC45; FLJ26304; ribosomal protein L15; RL15_HUMAN; RPL10; RPL15; RPLY10; RPYL10;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL15, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61313 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
K5 Acetylation
K5 Ubiquitination
S34 Phosphorylation
K47 Ubiquitination
K54 Ubiquitination
K56 Ubiquitination
Y59 Phosphorylation
Y62 Phosphorylation
K77 Ubiquitination
Y81 Phosphorylation
K83 Acetylation
K83 Ubiquitination
K93 Acetylation
K93 Ubiquitination
S97 Phosphorylation
S100 Phosphorylation
R105 Methylation
T126 Phosphorylation
Y127 Phosphorylation
K140 Sumoylation
K140 Ubiquitination
K153 Acetylation
K153 Ubiquitination
K157 Ubiquitination
R162 Methylation
K179 Acetylation
K179 Ubiquitination
T183 Phosphorylation
S187 Phosphorylation
T197 Phosphorylation
R202 Methylation

Research Backgrounds

Subcellular Location:

Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with IFIT1 (via TPR repeats 1-4).

Family&Domains:

Belongs to the eukaryotic ribosomal protein eL15 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.