Product: RPL12 Antibody
Catalog: DF3697
Description: Rabbit polyclonal antibody to RPL12
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 18 KD; 18kD(Calculated).
Uniprot: P30050
RRID: AB_2836061

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL12 Antibody detects endogenous levels of total RPL12.
RRID:
AB_2836061
Cite Format: Affinity Biosciences Cat# DF3697, RRID:AB_2836061.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L12; L12; Ribosomal protein L12; RL12_HUMAN; rpl12;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL12, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECPAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
78
Zebrafish
67
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30050 As Substrate

Site PTM Type Enzyme
Y14 Phosphorylation
C17 S-Nitrosylation
K31 Ubiquitination
S38 Phosphorylation P24941 (CDK2)
K40 Acetylation
K40 Ubiquitination
K41 Ubiquitination
K48 Ubiquitination
K54 Acetylation
K54 Ubiquitination
K61 Ubiquitination
R67 Methylation
K83 Acetylation
K83 Ubiquitination
K99 Ubiquitination
S101 Phosphorylation
T105 Phosphorylation
R114 Methylation
S126 Phosphorylation
T128 Phosphorylation
K130 Ubiquitination
T135 Phosphorylation
S138 Phosphorylation
C141 S-Nitrosylation
C162 S-Nitrosylation
S165 Phosphorylation

Research Backgrounds

Function:

Binds directly to 26S ribosomal RNA.

Family&Domains:

Belongs to the universal ribosomal protein uL11 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.