Product: RPL10L Antibody
Catalog: DF3695
Description: Rabbit polyclonal antibody to RPL10L
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 20 KD; 25kD(Calculated).
Uniprot: Q96L21
RRID: AB_2836059

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(90%), Bovine(80%), Sheep(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
RPL10L Antibody detects endogenous levels of total RPL10L.
RRID:
AB_2836059
Cite Format: Affinity Biosciences Cat# DF3695, RRID:AB_2836059.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L10 like; FLJ27353; Ribosomal protein L10 like; Ribosomal protein L10 like protein; RPL10_5_1358;

Immunogens

Immunogen:

A synthesized peptide derived from human RPL10L, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q96L21 RL10L_HUMAN:

Testis specific.

Sequence:
MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLGGHMVSDEYEQLSSEALEAARICANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNEEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAKKCLIPDGCGVKYVPSHGPLDKWRVLHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Bovine
80
Sheep
80
Dog
80
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96L21 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
K13 Ubiquitination
Y17 Phosphorylation
K19 Ubiquitination
R24 Methylation
K30 Sumoylation
K30 Ubiquitination
R32 Methylation
S54 Phosphorylation
Y57 Phosphorylation
S61 Phosphorylation
K74 Acetylation
K74 Sumoylation
K74 Ubiquitination
Y75 Phosphorylation
K101 Ubiquitination
S104 Phosphorylation
R110 Methylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
T125 Phosphorylation
R153 Methylation
K156 Ubiquitination
K158 Sumoylation
K158 Ubiquitination
K164 Acetylation
K164 Ubiquitination
K170 Methylation
K170 Sumoylation
K170 Ubiquitination
K208 Acetylation
K208 Ubiquitination

Research Backgrounds

Function:

May play a role in compensating for the inactivated X-linked gene during spermatogenesis.

Tissue Specificity:

Testis specific.

Family&Domains:

Belongs to the universal ribosomal protein uL16 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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