MAN1B1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using MAN1B1 Antibody.

Product:	MAN1B1 Antibody
Catalog:	AF0386
Description:	Rabbit polyclonal antibody to MAN1B1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	80kDa; 80kD(Calculated).
Uniprot:	Q9UKM7
RRID:	AB_2833542

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MAN1B1 Antibody detects endogenous levels of total MAN1B1.

RRID:

AB_2833542
Cite Format: Affinity Biosciences Cat# AF0386, RRID:AB_2833542.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha 1 2 mannosidase; Endoplasmic reticulum alpha mannosidase 1; Endoplasmic reticulum mannosyl oligosaccharide 1 2 alpha mannosidase 1; Endoplasmic reticulum mannosyl oligosaccharide 1 2 alpha mannosidase; ER alpha 1 2 mannosidase; Man9GlcNAc2 specific processing alpha mannosidase; MANA ER; Mannosidase alpha class 1B member 1;

Immunogens

Immunogen:

Uniprot:

Q9UKM7(MA1B1_HUMAN) >>Visit HPA database.

Gene(ID):

MAN1B1(11253)

Expression:

Q9UKM7 MA1B1_HUMAN:

Widely expressed.

Description:

MAN1B1 Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC)

Sequence:

Q9UKM7 MA1B1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA

PTMs - Q9UKM7 As Substrate

Q9UKM7

Site	PTM Type	Source
T21	Phosphorylation	Uniprot
Y60	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K129	Ubiquitination	Uniprot
K156	Ubiquitination	Uniprot
S174	O-Glycosylation	Uniprot
T204	O-Glycosylation	Uniprot
T219	O-Glycosylation	Uniprot
S223	O-Glycosylation	Uniprot
T230	O-Glycosylation	Uniprot
T239	O-Glycosylation	Uniprot
T242	O-Glycosylation	Uniprot
K275	Ubiquitination	Uniprot
S341	Phosphorylation	Uniprot
K354	Acetylation	Uniprot
K371	Ubiquitination	Uniprot
T381	O-Glycosylation	Uniprot
K434	Ubiquitination	Uniprot
Y487	Phosphorylation	Uniprot
T508	O-Glycosylation	Uniprot
T565	Phosphorylation	Uniprot
Y577	Phosphorylation	Uniprot
K588	Ubiquitination	Uniprot
K649	Ubiquitination	Uniprot

Research Backgrounds

Q9UKM7_MA1B1_HUMAN

Function:

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the glycosyl hydrolase 47 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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MAN1B1 Antibody - #AF0386