Product: RPS8 Antibody
Catalog: DF3689
Description: Rabbit polyclonal antibody to RPS8
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 30 KD; 24kD(Calculated).
Uniprot: P62241
RRID: AB_2836053

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RPS8 Antibody detects endogenous levels of total RPS8.
RRID:
AB_2836053
Cite Format: Affinity Biosciences Cat# DF3689, RRID:AB_2836053.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S8; OK/SW-cl.83; OTTHUMP00000010160; OTTHUMP00000010161; Ribosomal protein S8; RPS8; RS8_HUMAN; S8;

Immunogens

Immunogen:

A synthesized peptide derived from human RPS8, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62241 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S4 Phosphorylation
K23 Acetylation
K24 Acetylation
K26 Sumoylation
K26 Ubiquitination
Y27 Phosphorylation
T36 Phosphorylation
K37 Ubiquitination
S69 Phosphorylation
C71 S-Nitrosylation
C72 S-Nitrosylation
T76 Phosphorylation
Y83 Phosphorylation
S86 Phosphorylation
T93 Phosphorylation
K98 Ubiquitination
C100 S-Nitrosylation
S106 Phosphorylation
T107 Phosphorylation
Y109 Phosphorylation
R110 Methylation
Y113 Phosphorylation
Y117 Phosphorylation
K125 Acetylation
K128 Acetylation
K128 Sumoylation
K128 Ubiquitination
T130 Phosphorylation
K139 Ubiquitination
S142 Phosphorylation
K157 Sumoylation
K157 Ubiquitination
S159 Phosphorylation
S160 Phosphorylation
K170 Ubiquitination
R184 Methylation
Y188 Phosphorylation
K193 Ubiquitination
Y198 Phosphorylation
R200 Methylation

Research Backgrounds

Subcellular Location:

Cytoplasm. Membrane>Lipid-anchor.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS8 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

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For Research Use Only.
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