Product: RPS7 Antibody
Catalog: DF3688
Description: Rabbit polyclonal antibody to RPS7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 22 KD; 22kD(Calculated).
Uniprot: P62081
RRID: AB_2836052

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS7 Antibody detects endogenous levels of total RPS7.
RRID:
AB_2836052
Cite Format: Affinity Biosciences Cat# DF3688, RRID:AB_2836052.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S7; DBA8; Ribosomal protein S7; RPS 7; rps7; RS7_HUMAN; S7;

Immunogens

Immunogen:

A synthesized peptide derived from human RPS7, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62081 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
K10 Ubiquitination
K15 Ubiquitination
S24 Phosphorylation
K37 Acetylation
K37 Ubiquitination
K49 Ubiquitination
K58 Ubiquitination
K70 Acetylation
K70 Ubiquitination
K74 Acetylation
K74 Ubiquitination
K85 Sumoylation
K85 Ubiquitination
K86 Sumoylation
K86 Ubiquitination
S88 Phosphorylation
K90 Sumoylation
K90 Ubiquitination
S119 Phosphorylation
T121 Phosphorylation
T123 Phosphorylation
S137 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K155 Acetylation
K155 Ubiquitination
K160 Ubiquitination
K169 Acetylation
K169 Ubiquitination
S174 Phosphorylation
Y177 Phosphorylation
K178 Acetylation
K178 Methylation
K178 Ubiquitination
K179 Ubiquitination
K183 Sumoylation
K183 Ubiquitination

Research Backgrounds

Function:

Required for rRNA maturation.

PTMs:

Phosphorylated by NEK6.

Ubiquitinated. Deubiquitinated by DESI2, leading to its stabilization.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with NEK6 in the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds IPO9 with high affinity. Interacts with NEK6. Interacts with DESI2.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS7 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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For Research Use Only.
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