Product: RPS18 Antibody
Catalog: DF3679
Description: Rabbit polyclonal antibody to RPS18
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 17 KD; 18kD(Calculated).
Uniprot: P62269
RRID: AB_2836051

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS18 Antibody detects endogenous levels of total RPS18.
RRID:
AB_2836051
Cite Format: Affinity Biosciences Cat# DF3679, RRID:AB_2836051.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S18; HKE3; KE 3; Ke-3; Ke3; Rhabdomyosarcoma antigen MU-RMS-40.21; Ribosomal protein S18; rps18; RS18_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human RPS18, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Horse
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62269 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K8 Ubiquitination
R23 Methylation
K25 Ubiquitination
K34 Ubiquitination
Y40 Phosphorylation
R46 Methylation
K47 Ubiquitination
K54 Acetylation
K54 Ubiquitination
R55 Methylation
T60 Phosphorylation
K78 Acetylation
K78 Sumoylation
K78 Ubiquitination
K88 Sumoylation
K88 Ubiquitination
K94 Acetylation
K94 Sumoylation
K94 Ubiquitination
Y95 Phosphorylation
S96 Phosphorylation
K106 Acetylation
K106 Sumoylation
K106 Ubiquitination
R113 Methylation

Research Backgrounds

Function:

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the universal ribosomal protein uS13 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

References

1). Identification of Bone Morphometric Protein-Related Hub Genes and Construction of a Transcriptional Regulatory Network in Patients With Ossification of the Ligamentum Flavum. Spine (PubMed: 37384872) [IF=3.0]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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