Product: MRPL40 Antibody
Catalog: DF3669
Description: Rabbit polyclonal antibody to MRPL40
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 24 KD; 24kD(Calculated).
Uniprot: Q9NQ50
RRID: AB_2836041

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(88%), Zebrafish(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MRPL40 Antibody detects endogenous levels of total MRPL40.
RRID:
AB_2836041
Cite Format: Affinity Biosciences Cat# DF3669, RRID:AB_2836041.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39S ribosomal protein L40; 39S ribosomal protein L40 mitochondrial; 39S ribosomal protein L40, mitochondrial precursor; FLJ41774; L40mt; MGC9400; mitochondrial; Mitochondrial ribosomal protein L40; MRP 40; MRP L22; MRP-L40; MRP40; MRPL22; MRPL40; NLVCF; Nuclear localization signal containing protein deleted in velocardiofacial syndrome; Nuclear localization signal deleted in velocardiofacial syndrome; Nuclear localization signal-containing protein deleted in velocardiofacial syndrome; RM40_HUMAN; Up-regulated in metastasis; URIM;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NQ50 RM40_HUMAN:

Ubiquitous.

Sequence:
MTASVLRSISLALRPTSGLLGTWQTQLRETHQRASLLSFWELIPMRSEPLRKKKKVDPKKDQEAKERLKRKIRKLEKATQELIPIEDFITPLKFLDKARERPQVELTFEETERRALLLKKWSLYKQQERKMERDTIRAMLEAQQEALEELQLESPKLHAEAIKRDPNLFPFEKEGPHYTPPIPNYQPPEGRYNDITKVYTQVEFKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Xenopus
100
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Zebrafish
88
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQ50 As Substrate

Site PTM Type Enzyme
K77 Ubiquitination
T90 Phosphorylation
S154 Phosphorylation
Y199 Phosphorylation

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. mL40 binds to the major groove of the anticodon stem of mt-tRNA(Val) in the central protuberance.

Family&Domains:

Belongs to the mitochondrion-specific ribosomal protein mL40 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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