Product: MRPL32 Antibody
Catalog: DF3667
Description: Rabbit polyclonal antibody to MRPL32
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 21 KD; 21kD(Calculated).
Uniprot: Q9BYC8
RRID: AB_2836039

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(91%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MRPL32 Antibody detects endogenous levels of total MRPL32.
RRID:
AB_2836039
Cite Format: Affinity Biosciences Cat# DF3667, RRID:AB_2836039.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39S ribosomal protein L32; 39S ribosomal protein L32 mitochondrial; bMRP 59b; HSPC283; L32mt; mitochondrial; Mitochondrial ribosomal protein L32; MRP-L32; mRpL32; RM32_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWGPALAVQGPAMFTEPANDTSGSKENSSLLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQIGKQEGGPFKAPTIETVVLYTGETPSEQDQGKRIIERDRKRPSWFTQN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Pig
91
Bovine
91
Zebrafish
64
Horse
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYC8 As Substrate

Site PTM Type Enzyme
S34 Phosphorylation
T153 Phosphorylation
T164 Phosphorylation
K172 Ubiquitination
S183 Phosphorylation
T186 Phosphorylation

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. bL32m has a zinc binding site.

Family&Domains:

Belongs to the bacterial ribosomal protein bL32 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

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