Product: MRPL14 Antibody
Catalog: DF3659
Description: Rabbit polyclonal antibody to MRPL14
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.: 16 KD; 16kD(Calculated).
Uniprot: Q6P1L8
RRID: AB_2836031

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(83%), Horse(83%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
MRPL14 Antibody detects endogenous levels of total MRPL14.
RRID:
AB_2836031
Cite Format: Affinity Biosciences Cat# DF3659, RRID:AB_2836031.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110006I11Rik; 39S ribosomal protein L14; 39S ribosomal protein L14 mitochondrial; 39S ribosomal protein L32; AI846816; L14mt; L32mt; mitochondrial; MRP L14; MRP L32; MRP-L14; MRP-L32; mrpl14; RM14_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAFFTGLWGPFTCVSRVLSHHCFSTTGSLSAIQKMTRVRVVDNSALGNSPYHRAPRCIHVYKKNGVGKVGDQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAIAQNFV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Bovine
83
Sheep
83
Dog
83
Zebrafish
83
Pig
75
Xenopus
75
Chicken
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P1L8 As Substrate

Site PTM Type Enzyme
S49 Phosphorylation
Y51 Phosphorylation
T121 Phosphorylation
T125 Phosphorylation

Research Backgrounds

Function:

Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation (Probable).

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. Interacts with MALSU1.

Family&Domains:

Belongs to the universal ribosomal protein uL14 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

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For Research Use Only.
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