MRPS9 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A549 cells, using MRPS9 antibody.

Product:	MRPS9 Antibody
Catalog:	DF3652
Description:	Rabbit polyclonal antibody to MRPS9
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	40 KD; 46kD(Calculated).
Uniprot:	P82933
RRID:	AB_2836024

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Zebrafish(93%), Bovine(86%), Horse(93%), Sheep(86%), Rabbit(93%), Dog(86%)

Clonality:

Polyclonal

Specificity:

MRPS9 Antibody detects endogenous levels of total MRPS9.

RRID:

AB_2836024
Cite Format: Affinity Biosciences Cat# DF3652, RRID:AB_2836024.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

28S ribosomal protein S9; 28S ribosomal protein S9 mitochondrial; 28S ribosomal protein S9 mitochondrial precursor; mitochondrial; mitochondrial ribosomal protein S9; MRP S9; MRP-S9; MRPS9; RPMS9; RT09_HUMAN; S9mt;

Immunogens

Immunogen:

Uniprot:

P82933(RT09_HUMAN) >>Visit HPA database.

Gene(ID):

MRPS9(64965)

Sequence:

P82933 RT09_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPCVSYGGAVSYRLLLWGRGSLARKQGLWKTAAPELQTNVRSQILRLRHTAFVIPKKNVPTSKRETYTEDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDIDRAIAYLFPSGLFEKRARPVMKHPEQIFPRQRAIQWGEDGRPFHYLFYTGKQSYYSLMHDVYGMLLNLEKHQSHLQAKSLLPEKTVTRDVIGSRWLIKEELEEMLVEKLSDLDYMQFIRLLEKLLTSQCGAAEEEFVQRFRRSVTLESKKQLIEPVQYDEQGMAFSKSEGKRKTAKAEAIVYKHGSGRIKVNGIDYQLYFPITQDREQLMFPFHFVDRLGKHDVTCTVSGGGRSAQAGAIRLAMAKALCSFVTEDEVEWMRQAGLLTTDPRVRERKKPGQEGARRKFTWKKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

Horse

Rabbit

Pig

Bovine

Sheep

Dog

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P82933 As Substrate

P82933

Site	PTM Type	Source
T52	Phosphorylation	Uniprot
Y69	Phosphorylation	Uniprot
K75	Ubiquitination	Uniprot
K76	Ubiquitination	Uniprot
K85	Ubiquitination	Uniprot
Y110	Phosphorylation	Uniprot
S114	Phosphorylation	Uniprot
K119	Ubiquitination	Uniprot
K126	Ubiquitination	Uniprot
K182	Ubiquitination	Uniprot
K188	Ubiquitination	Uniprot
S214	Phosphorylation	Uniprot
Y218	Phosphorylation	Uniprot
K253	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot
Y262	Phosphorylation	Uniprot
K271	Ubiquitination	Uniprot
K280	Ubiquitination	Uniprot
K287	Acetylation	Uniprot
K287	Ubiquitination	Uniprot
K325	Acetylation	Uniprot
K325	Ubiquitination	Uniprot
C330	S-Nitrosylation	Uniprot
K380	Acetylation	Uniprot
T392	Phosphorylation	Uniprot

Research Backgrounds

P82933_RT09_HUMAN

Subcellular Location:

Mitochondrion.

Subunit Structure:

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the universal ribosomal protein uS9 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

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For Research Use Only.
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MRPS9 Antibody - #DF3652