PSMD3 Antibody - #DF3645

Fig. 1 PSMD3 was upregulated in lung cancer and high PSMD3 indicates poor prognosis. (A) The expression of PSMD3 in LUAD and LUSC used samples from TCGA. (B) Kaplan-Meier analysis showed the low OS in high-PSMD3 TPM. (C) Representative images of IHC on PSMD3 expression in paired tumor and nearby noncancerous tissues from two typical patients. The scale bar stands for 100 μm. (D) The protein level of PSMD3 in 10 pairs normal versus LC tissue samples using western blot. GADPH served as internal control. N: Normal; T: tumor. (E) The protein level of PSMD3 in five LC cell lines and a normal lung cell. GADPH was internal control.

Fig. 2 PSMD3 promotes cell proliferation in LC. (A-B) The protein levels of PSMD3 in two LC cell lines transfected with siPSMD3#1, siPSMD3#2, and siCtrl or pcDNA3.1-PSMD3, and Ctrl were determined using western blot. (C-D) Cell viability after PSMD3 knockdown of overexpression at 24, 48, 72 and 96 h were assessed by CCK-8 assay. (E-F) Cell colonies at 14 days in LC cells. (G-H) Cell apoptosis at 24 h after being treated with the suggested siRNAs and plasmids were assessed by flow cytometry. (I) Tumor size in nude mice between shCtrl and shPSMD3 groups were sacrificed at day 35. Images of the xenograft mice with tumors are displayed. (J) The measurement of tumor volumes of nude mice among three groups was made with digital calipers at day 7, 14, 21, 28, and 35. (K) The weight of xenograft tumors was measured among three mice groups at day 35. (L) Images of IHC staining of PSMD3 between two group tumor tissues.

Figure 8. rSS1GFP infection affects the expression of cellular translation, posttranslational modification and trafficking-associated proteins. (A) The heatmap of representative 20 DEPs related to “Translation, ribosomal structure and biogenesis” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Translation, ribosomal structure and biogenesis” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “Posttranslational modification, protein turnover, chaperones” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “Posttranslational modification, protein turnover, chaperones” are analyzed by the STRING software. (E) The heatmap of representative 20 DEPs related to “Intracellular trafficking, secretion, and vesicular transport” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (F) The protein-protein interactions of the DEPs related to “Intracellular trafficking, secretion, and vesicular transport” are analyzed by the STRING software. (G) The mRNA expression levels of six selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (H) The protein expression levels of six DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of six DEPs were compared with the control GAPDH expression.