PSMD12 Antibody - #DF3644

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Product: PSMD12 Antibody
Catalog: DF3644
Description: Rabbit polyclonal antibody to PSMD12
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 50 KD; 53kD(Calculated).
Uniprot: O00232
RRID: AB_2836016

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
PSMD12 Antibody detects endogenous levels of total PSMD12.
RRID:
AB_2836016
Cite Format: Affinity Biosciences Cat# DF3644, RRID:AB_2836016.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit p55; 26S proteasome regulatory subunit RPN5; MGC75406; p55; proteasome (prosome, macropain) 26S subunit, non ATPase12; Proteasome 26S non ATPase subunit 12 isoform 2; PSD12_HUMAN; PSMD12; Rpn5;

Immunogens

Immunogen:
Uniprot:

O00232(PSD12_HUMAN) >>Visit HPA database.

Gene(ID):
PSMD12(5718)
Sequence:
MADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAVVKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPIKLRLIDTLRMVTEGKIYVEIERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKRWKDLKNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGIINFQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
82
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00232 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y20 Phosphorylation
S21 Phosphorylation
K33 Ubiquitination
K36 Ubiquitination
K52 Ubiquitination
K92 Sumoylation
K92 Ubiquitination
K98 Ubiquitination
K103 Ubiquitination
Y111 Phosphorylation
K121 Ubiquitination
Y137 Phosphorylation
K147 Ubiquitination
K153 Ubiquitination
K160 Ubiquitination
K179 Ubiquitination
K212 Acetylation
K212 Ubiquitination
T219 Phosphorylation
K221 Acetylation
K221 Ubiquitination
Y227 Phosphorylation
C255 S-Nitrosylation
K262 Ubiquitination
K295 Ubiquitination
K301 Ubiquitination
K330 Ubiquitination
S332 Phosphorylation
S335 Phosphorylation
S343 Phosphorylation
K349 Ubiquitination
K368 Acetylation
K368 Ubiquitination
Y369 Phosphorylation
Y370 Phosphorylation
K419 Ubiquitination
K431 Ubiquitination
S434 Phosphorylation
S437 Phosphorylation
K441 Ubiquitination
K448 Acetylation
K448 Ubiquitination

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD12, a base containing 6 ATPases and few additional components. Interacts with ERCC6.

Family&Domains:

Belongs to the proteasome subunit p55 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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