PSMD11 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells using PSMD11 antibody.

DF3643 at 1/100 staining mouse muscle tissue by IHC-P.

Product:	PSMD11 Antibody
Catalog:	DF3643
Description:	Rabbit polyclonal antibody to PSMD11
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	42 KD; 47kD(Calculated).
Uniprot:	O00231
RRID:	AB_2836015

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

PSMD11 Antibody detects endogenous levels of total PSMD11.

RRID:

AB_2836015
Cite Format: Affinity Biosciences Cat# DF3643, RRID:AB_2836015.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; MGC3844; p44.5; protease 26S, subunit, 9; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; proteasome 26S subunit, non-ATPase, 11; PSD11_HUMAN; PSMD 11; PSMD11; Rpn6; S9;

Immunogens

Immunogen:

Uniprot:

O00231(PSD11_HUMAN) >>Visit HPA database.

Gene(ID):

PSMD11(5717)

Expression:

O00231 PSD11_HUMAN:

Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.

Sequence:

O00231 PSD11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00231 As Substrate

O00231

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
R11	Methylation		Uniprot
S14	Phosphorylation	P17612 (PRKACA)	Uniprot
R20	Methylation		Uniprot
S23	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
K32	Ubiquitination		Uniprot
K46	Ubiquitination		Uniprot
S49	Phosphorylation		Uniprot
S55	Phosphorylation		Uniprot
K59	Ubiquitination		Uniprot
T60	Phosphorylation		Uniprot
K71	Ubiquitination		Uniprot
Y72	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
K82	Ubiquitination		Uniprot
Y137	Phosphorylation		Uniprot
K141	Acetylation		Uniprot
K141	Ubiquitination		Uniprot
S151	Phosphorylation		Uniprot
K175	Ubiquitination		Uniprot
S181	Phosphorylation		Uniprot
K185	Ubiquitination		Uniprot
Y201	Phosphorylation		Uniprot
C202	S-Nitrosylation		Uniprot
K205	Ubiquitination		Uniprot
T209	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
K223	Ubiquitination		Uniprot
K246	Ubiquitination		Uniprot
K252	Ubiquitination		Uniprot
S272	Phosphorylation		Uniprot
K274	Ubiquitination		Uniprot
T284	Phosphorylation		Uniprot
K288	Ubiquitination		Uniprot
C289	S-Nitrosylation		Uniprot
K295	Ubiquitination		Uniprot
S298	Phosphorylation		Uniprot
K304	Methylation		Uniprot
K304	Ubiquitination		Uniprot
S320	Phosphorylation		Uniprot
K325	Ubiquitination		Uniprot
K358	Ubiquitination		Uniprot
K364	Ubiquitination		Uniprot
K372	Ubiquitination		Uniprot
T396	Phosphorylation		Uniprot
Y397	Phosphorylation		Uniprot
S413	Phosphorylation		Uniprot
Y415	Phosphorylation		Uniprot
K417	Acetylation		Uniprot
K417	Ubiquitination		Uniprot

Research Backgrounds

O00231_PSD11_HUMAN

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

PTMs:

Phosphorylated by AMPK.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol.

Tissue Specificity:

Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD11, a base containing 6 ATPases and few additional components.

Family&Domains:

Belongs to the proteasome subunit S9 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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For Research Use Only.
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PSMD11 Antibody - #DF3643