EFTUD2 Antibody - #DF3639

(2)  
Product: EFTUD2 Antibody
Catalog: DF3639
Description: Rabbit polyclonal antibody to EFTUD2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 109 KD; 109kD(Calculated).
Uniprot: Q15029
RRID: AB_2836011

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EFTUD2 Antibody detects endogenous levels of total EFTUD2.
RRID:
AB_2836011
Cite Format: Affinity Biosciences Cat# DF3639, RRID:AB_2836011.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

116 kDa; 116 kDa U5 small nuclear ribonucleoprotein component; EFTUD2; Elongation factor Tu GTP binding domain containing 2; Elongation factor Tu GTP-binding domain-containing protein 2; hSNU114; MFDGA; MFDM; SNRNP116; Snrp116; Snu114; SNU114 homolog; U5 116KD; U5 small nuclear ribonucleoprotein component; U5 snRNP specific protein, 116 kD; U5 snRNP specific protein, 116 kDa; U5 snRNP-specific protein; U5-116 kDa; U5-116KD; U5S1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q15029(U5S1_HUMAN) >>Visit HPA database.

Gene(ID):
EFTUD2(9343)
Sequence:
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15029 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
Y6 Phosphorylation
S19 Phosphorylation
T30 Phosphorylation
K64 Sumoylation
K64 Ubiquitination
Y73 Phosphorylation
T79 Phosphorylation
T86 Phosphorylation
T90 Phosphorylation
K95 Ubiquitination
K159 Ubiquitination
Y167 Phosphorylation
T168 Phosphorylation
K182 Ubiquitination
T184 Phosphorylation
K244 Ubiquitination
Y336 Phosphorylation
K341 Ubiquitination
Y348 Phosphorylation
K352 Ubiquitination
T353 Phosphorylation
K405 Ubiquitination
K409 Ubiquitination
S459 Phosphorylation
S466 Phosphorylation
S498 Phosphorylation
K508 Ubiquitination
S533 Phosphorylation
K561 Ubiquitination
T562 Phosphorylation
T564 Phosphorylation
T566 Phosphorylation
R569 Methylation
K581 Acetylation
K581 Ubiquitination
S598 Phosphorylation
K602 Ubiquitination
K612 Ubiquitination
K646 Ubiquitination
S649 Phosphorylation
K673 Ubiquitination
K681 Ubiquitination
K684 Ubiquitination
K694 Ubiquitination
K713 Ubiquitination
K721 Ubiquitination
T740 Phosphorylation
T749 Phosphorylation
S752 Phosphorylation
K756 Ubiquitination
K763 Ubiquitination
K788 Ubiquitination
K790 Acetylation
K790 Ubiquitination
R803 Methylation
T811 Phosphorylation
R813 Methylation
T824 Phosphorylation
S866 Phosphorylation
T885 Phosphorylation
K936 Ubiquitination
K941 Ubiquitination
S944 Phosphorylation
S948 Phosphorylation
S950 Phosphorylation
K951 Ubiquitination
K963 Ubiquitination

Research Backgrounds

Function:

Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the pre-catalytic, catalytic and post-catalytic spliceosome complexes. Interacts with ERBB4 and PRPF8. Interacts with PIH1D1. Interacts with RPAP3 and URI1 in a ZNHIT2-dependent manner.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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