ORC1L Antibody - #DF3637

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Product: ORC1L Antibody
Catalog: DF3637
Description: Rabbit polyclonal antibody to ORC1L
Application: WB IF/ICC
Reactivity: Human
Prediction: Rabbit
Mol.Wt.: 100,120 KD; 97kD(Calculated).
Uniprot: Q13415
RRID: AB_2836009

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(91%)
Clonality:
Polyclonal
Specificity:
ORC1L Antibody detects endogenous levels of total ORC1L.
RRID:
AB_2836009
Cite Format: Affinity Biosciences Cat# DF3637, RRID:AB_2836009.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 (yeast homolog) like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like (yeast); PARC1; Replication control protein 1;

Immunogens

Immunogen:
Uniprot:

Q13415(ORC1_HUMAN) >>Visit HPA database.

Gene(ID):
ORC1(4998)
Sequence:
MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSSSDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRNDLLLRVRLNVSQDDVLYALKDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Horse
73
Dog
73
Pig
70
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13415 As Substrate

Site PTM Type Enzyme
K25 Sumoylation
K25 Ubiquitination
K81 Ubiquitination
K99 Ubiquitination
K149 Ubiquitination
K152 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
K178 Ubiquitination
S182 Phosphorylation
S194 Phosphorylation
K195 Ubiquitination
S196 Phosphorylation
S199 Phosphorylation
S201 Phosphorylation
T203 Phosphorylation
K210 Ubiquitination
S217 Phosphorylation
S221 Phosphorylation
T224 Phosphorylation
T226 Phosphorylation
T230 Phosphorylation
S255 Phosphorylation
S258 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S269 Phosphorylation
S273 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S275 Phosphorylation
T285 Phosphorylation
S287 Phosphorylation
S311 Phosphorylation
K326 Acetylation
T337 Phosphorylation
S340 Phosphorylation
S350 Phosphorylation
K364 Acetylation
T373 Phosphorylation
S374 Phosphorylation
T375 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S383 Phosphorylation
S384 Phosphorylation
T387 Phosphorylation
S417 Phosphorylation
S419 Phosphorylation
S420 Phosphorylation
S421 Phosphorylation
S427 Phosphorylation
K448 Methylation
K448 Ubiquitination
S449 Phosphorylation
S450 Phosphorylation
T453 Phosphorylation
T455 Phosphorylation
K456 Ubiquitination
S461 Phosphorylation
S476 Phosphorylation
S478 Phosphorylation
K540 Ubiquitination
K586 Ubiquitination
K591 Ubiquitination
K603 Ubiquitination
S610 Phosphorylation
K698 Ubiquitination
K711 Ubiquitination
S850 Phosphorylation
K859 Ubiquitination

Research Backgrounds

Function:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

PTMs:

Phosphorylated during mitosis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated.

Family&Domains:

The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

Belongs to the ORC1 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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