T3JAM Antibody - #DF3633
(6)
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Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
T3JAM Antibody detects endogenous levels of total T3JAM.
RRID:
AB_2836005
Cite Format: Affinity Biosciences Cat# DF3633, RRID:AB_2836005.
Cite Format: Affinity Biosciences Cat# DF3633, RRID:AB_2836005.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
T3JAM; TRAF3 interacting JNK activating modulator; TRAF3 interacting protein 3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9Y228 T3JAM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MISPDPRPSPGLARWAESYEAKCERRQEIRESRRCRPNVTTCRQVGKTLRIQQREQLQRARLQQFFRRRNLELEEKGKAQHPQAREQGPSRRPGQVTVLKEPLSCARRISSPREQVTGTSSEVFPAQHPPPSGICRDLSDHLSSQAGGLPPQDTPIKKPPKHHRGTQTKAEGPTIKNDASQQTNYGVAVLDKEIIQLSDYLKEALQRELVLKQKMVILQDLLSTLIQASDSSWKGQLNEDKLKGKLRSLENQLYTCTQKYSPWGMKKVLLEMEDQKNSYEQKAKESLQKVLEEKMNAEQQLQSTQRSLALAEQKCEEWRSQYEALKEDWRTLGTQHRELESQLHVLQSKLQGADSRDLQMNQALRFLENEHQQLQAKIECLQGDRDLCSLDTQDLQDQLKRSEAEKLTLVTRVQQLQGLLQNQSLQLQEQEKLLTKKDQALPVWSPKSFPNEVEPEGTGKEKDWDLRDQLQKKTLQLQAKEKECRELHSELDNLSDEYLSCLRKLQHCREELNQSQQLPPRRQCGRWLPVLMVVIAAALAVFLANKDNLMI
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Chicken
80
Xenopus
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y228 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| Y254 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
May function as an adapter molecule that regulates TRAF3-mediated JNK activation.
Subcellular Location:
Membrane>Single-pass type IV membrane protein.
Subunit Structure:
Binds to the isoleucine zipper of TRAF3 via its coiled-coil domain.
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For Research Use Only.
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