RAD54 Antibody - #DF3631

(2)  
Product: RAD54 Antibody
Catalog: DF3631
Description: Rabbit polyclonal antibody to RAD54
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 85 KD; 84kD(Calculated).
Uniprot: Q92698
RRID: AB_2836003

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
RAD54 Antibody detects endogenous levels of total RAD54.
RRID:
AB_2836003
Cite Format: Affinity Biosciences Cat# DF3631, RRID:AB_2836003.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair and recombination protein RAD54 like; DNA repair and recombination protein RAD54-like; hHR 54; hHR54; HR 54; HR54; hRAD 54; hRAD54; RAD 54A; RAD 54L; RAD54 (S. cerevisiae) like; RAD54 homolog; RAD54 like (S. cerevisiae); RAD54 like; RAD54 like protein; RAD54_HUMAN; RAD54A; RAD54L;

Immunogens

Immunogen:
Uniprot:

Q92698(RAD54_HUMAN) >>Visit HPA database.

Gene(ID):
RAD54L(8438)
Sequence:
MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
82
Zebrafish
70
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92698 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K12 Acetylation
T31 Phosphorylation
S37 Phosphorylation
S38 Phosphorylation
S39 Phosphorylation
T41 Phosphorylation
S49 Phosphorylation
K53 Ubiquitination
K83 Ubiquitination
K115 Ubiquitination
K133 Ubiquitination
K138 Ubiquitination
K224 Ubiquitination
K232 Ubiquitination
S247 Phosphorylation
K248 Ubiquitination
K254 Ubiquitination
K302 Ubiquitination
T308 Phosphorylation
Y309 Phosphorylation
S314 Phosphorylation
K355 Ubiquitination
K363 Ubiquitination
S386 Phosphorylation
K402 Ubiquitination
K407 Ubiquitination
K425 Ubiquitination
K432 Ubiquitination
K441 Ubiquitination
K454 Ubiquitination
K455 Ubiquitination
Y503 Phosphorylation
S572 Phosphorylation
K630 Ubiquitination
K639 Ubiquitination
K662 Ubiquitination
S671 Phosphorylation

Research Backgrounds

Function:

Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via N-terminus) with RAD51.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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