PARP2 Antibody - #DF3625

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Product: PARP2 Antibody
Catalog: DF3625
Description: Rabbit polyclonal antibody to PARP2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 75 KD; 66kD(Calculated).
Uniprot: Q9UGN5
RRID: AB_2835997

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PARP2 Antibody detects endogenous levels of total PARP2.
RRID:
AB_2835997
Cite Format: Affinity Biosciences Cat# DF3625, RRID:AB_2835997.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosyltransferase like 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT 2; ADPRT-2; ADPRT2; ADPRTL 2; ADPRTL 3; ADPRTL2; ADPRTL3; ARTD2; hPARP 2; hPARP-2; hPARP2; NAD(+) ADP ribosyltransferase 2; NAD(+) ADP-ribosyltransferase 2; pADPRT 2; pADPRT-2; pADPRT2; PARP 2; PARP-2; PARP2; PARP2_HUMAN; Poly (ADP ribose) polymerase family member 2; Poly (ADP ribosyl) transferase like 2; poly (ADP-ribose) polymerase 2; Poly [ADP ribose] synthetase 2; Poly [ADP-ribose] polymerase 2; Poly(ADP ribose) synthetase; Poly[ADP-ribose] synthase 2;

Immunogens

Immunogen:
Uniprot:

Q9UGN5(PARP2_HUMAN) >>Visit HPA database.

Gene(ID):
PARP2(10038)
Expression:
Q9UGN5 PARP2_HUMAN:

Widely expressed, mainly in actively dividing tissues (PubMed:10364231). The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus (PubMed:10364231).

Sequence:
MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Zebrafish
80
Xenopus
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UGN5 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
T9 Phosphorylation
S20 Phosphorylation
S33 Phosphorylation
S34 Phosphorylation
S47 Phosphorylation
K91 Ubiquitination
K101 Ubiquitination
K104 Ubiquitination
Y115 Phosphorylation
K171 Ubiquitination
K173 Ubiquitination
K196 Ubiquitination
S226 Phosphorylation
K229 Ubiquitination
S232 Phosphorylation
K269 Ubiquitination
T271 Phosphorylation
K276 Ubiquitination
T317 Phosphorylation
S328 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
S383 Phosphorylation
T389 Phosphorylation
S430 Phosphorylation
S467 Phosphorylation
K469 Ubiquitination
K480 Ubiquitination
K512 Ubiquitination
S514 Phosphorylation
K516 Ubiquitination

Research Backgrounds

Function:

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.

PTMs:

Poly-ADP-ribosylated by PARP1.

Acetylation reduces DNA binding and enzymatic activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.

Subunit Structure:

Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LRIG3 (By similarity). Homo- and heterodimer with PARP1. Interacts weakly (via the PARP catalytic domain) with HPF1.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Genetic Information Processing > Replication and repair > Base excision repair.

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