MSH3 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from HUVEC cells, using MSH3 antibody.

Product:	MSH3 Antibody
Catalog:	DF3624
Description:	Rabbit polyclonal antibody to MSH3
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	127 KD; 127kD(Calculated).
Uniprot:	P20585
RRID:	AB_2835996

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

MSH3 Antibody detects endogenous levels of total MSH3.

RRID:

AB_2835996
Cite Format: Affinity Biosciences Cat# DF3624, RRID:AB_2835996.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Divergent upstream protein; DNA mismatch repair protein; DNA mismatch repair protein Msh 3; DNA mismatch repair protein MSH3; DUC 1; DUC1; DUG; DUP; hMSH3; MGC163306; MGC163308; Mismatch repair protein 1; MRP 1; MRP1; MSH 3; MSH3; MSH3_HUMAN; MutS homolog 3 (E. coli); MutS homolog 3;

Immunogens

Immunogen:

Uniprot:

P20585(MSH3_HUMAN) >>Visit HPA database.

Gene(ID):

MSH3(4437)

Sequence:

P20585 MSH3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKLDDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSSLQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIISGIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSLLWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYHKKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKTELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVKVGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKVAKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVALITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVPDFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQKWTEEFNMEETQTSLLH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

Rabbit

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20585 As Substrate

P20585

Site	PTM Type	Source
S14	Phosphorylation	Uniprot
S16	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
S33	Phosphorylation	Uniprot
K103	Acetylation	Uniprot
K107	Ubiquitination	Uniprot
S111	Phosphorylation	Uniprot
S116	Phosphorylation	Uniprot
K165	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot
S201	Phosphorylation	Uniprot
K215	Ubiquitination	Uniprot
Y229	Phosphorylation	Uniprot
Y236	Phosphorylation	Uniprot
K240	Ubiquitination	Uniprot
K308	Ubiquitination	Uniprot
K316	Methylation	Uniprot
K316	Ubiquitination	Uniprot
S337	Phosphorylation	Uniprot
T338	Phosphorylation	Uniprot
S520	Phosphorylation	Uniprot
T540	Phosphorylation	Uniprot
T541	Phosphorylation	Uniprot
K557	Sumoylation	Uniprot
K586	Ubiquitination	Uniprot
K695	Ubiquitination	Uniprot
K704	Ubiquitination	Uniprot
K713	Ubiquitination	Uniprot
K770	Ubiquitination	Uniprot
K775	Ubiquitination	Uniprot
K842	Ubiquitination	Uniprot
K902	Ubiquitination	Uniprot
T967	Phosphorylation	Uniprot
S968	Phosphorylation	Uniprot
T1054	Phosphorylation	Uniprot
Y1068	Phosphorylation	Uniprot
K1074	Ubiquitination	Uniprot
K1091	Ubiquitination	Uniprot
T1099	Phosphorylation	Uniprot
K1100	Ubiquitination	Uniprot

Research Backgrounds

P20585_MSH3_HUMAN

Function:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Subunit Structure:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Interacts with MCM9.

Family&Domains:

Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MSH3 Antibody - #DF3624