MLH3 Antibody - #DF3622
(4)
| Product: | MLH3 Antibody |
| Catalog: | DF3622 |
| Description: | Rabbit polyclonal antibody to MLH3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine |
| Mol.Wt.: | 164 KD; 164kD(Calculated). |
| Uniprot: | Q9UHC1 |
| RRID: | AB_2835994 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1;200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%)
Clonality:
Polyclonal
Specificity:
MLH3 Antibody detects endogenous levels of total MLH3.
RRID:
AB_2835994
Cite Format: Affinity Biosciences Cat# DF3622, RRID:AB_2835994.
Cite Format: Affinity Biosciences Cat# DF3622, RRID:AB_2835994.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DNA mismatch repair protein Mlh3; HNPCC 7; HNPCC; HNPCC7; MGC138372; Mismatch repair gene MLH 3; Mismatch repair gene MLH3; MLH 3; MLH3; MLH3_HUMAN; MutL homolog 3 (E. coli); MutL homolog 3; MutL protein homolog 3; S240II117;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q9UHC1 MLH3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIKCLSVEVQAKLRSGLAISSLGQCVEELALNSIDAEAKCVAVRVNMETFQVQVIDNGFGMGSDDVEKVGNRYFTSKCHSVQDLENPRFYGFRGEALANIADMASAVEISSKKNRTMKTFVKLFQSGKALKACEADVTRASAGTTVTVYNLFYQLPVRRKCMDPRLEFEKVRQRIEALSLMHPSISFSLRNDVSGSMVLQLPKTKDVCSRFCQIYGLGKSQKLREISFKYKEFELSGYISSEAHYNKNMQFLFVNKRLVLRTKLHKLIDFLLRKESIICKPKNGPTSRQMNSSLRHRSTPELYGIYVINVQCQFCEYDVCMEPAKTLIEFQNWDTLLFCIQEGVKMFLKQEKLFVELSGEDIKEFSEDNGFSLFDATLQKRVTSDERSNFQEACNNILDSYEMFNLQSKAVKRKTTAENVNTQSSRDSEATRKNTNDAFLYIYESGGPGHSKMTEPSLQNKDSSCSESKMLEQETIVASEAGENEKHKKSFLEHSSLENPCGTSLEMFLSPFQTPCHFEESGQDLEIWKESTTVNGMAANILKNNRIQNQPKRFKDATEVGCQPLPFATTLWGVHSAQTEKEKKKESSNCGRRNVFSYGRVKLCSTGFITHVVQNEKTKSTETEHSFKNYVRPGPTRAQETFGNRTRHSVETPDIKDLASTLSKESGQLPNKKNCRTNISYGLENEPTATYTMFSAFQEGSKKSQTDCILSDTSPSFPWYRHVSNDSRKTDKLIGFSKPIVRKKLSLSSQLGSLEKFKRQYGKVENPLDTEVEESNGVTTNLSLQVEPDILLKDKNRLENSDVCKITTMEHSDSDSSCQPASHILNSEKFPFSKDEDCLEQQMPSLRESPMTLKELSLFNRKPLDLEKSSESLASKLSRLKGSERETQTMGMMSRFNELPNSDSSRKDSKLCSVLTQDFCMLFNNKHEKTENGVIPTSDSATQDNSFNKNSKTHSNSNTTENCVISETPLVLPYNNSKVTGKDSDVLIRASEQQIGSLDSPSGMLMNPVEDATGDQNGICFQSEESKARACSETEESNTCCSDWQRHFDVALGRMVYVNKMTGLSTFIAPTEDIQAACTKDLTTVAVDVVLENGSQYRCQPFRSDLVLPFLPRARAERTVMRQDNRDTVDDTVSSESLQSLFSEWDNPVFARYPEVAVDVSSGQAESLAVKIHNILYPYRFTKGMIHSMQVLQQVDNKFIACLMSTKTEENGEAGGNLLVLVDQHAAHERIRLEQLIIDSYEKQQAQGSGRKKLLSSTLIPPLEITVTEEQRRLLWCYHKNLEDLGLEFVFPDTSDSLVLVGKVPLCFVEREANELRRGRSTVTKSIVEEFIREQLELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDGLSLQESCRLIEALSSCQLPFQCAHGRPSMLPLADIDHLEQEKQIKPNLTKLRKMAQAWRLFGKAECDTRQSLQQSMPPCEPP
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
91
Pig
82
Horse
73
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UHC1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K128 | Ubiquitination | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| T606 | Phosphorylation | Uniprot | |
| T610 | Phosphorylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| T690 | Phosphorylation | Uniprot | |
| Y691 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| K876 | Ubiquitination | Uniprot | |
| K881 | Acetylation | Uniprot | |
| S883 | Phosphorylation | Uniprot | |
| K982 | Ubiquitination | Uniprot | |
| T1062 | Phosphorylation | Uniprot | |
| T1128 | Phosphorylation | Uniprot | |
| S1188 | Phosphorylation | Uniprot | |
| T1208 | Phosphorylation | Uniprot | |
| K1243 | Ubiquitination | Uniprot | |
| S1256 | Phosphorylation | Uniprot | |
| S1257 | Phosphorylation | Uniprot | |
| T1258 | Phosphorylation | Uniprot | |
| K1413 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Probably involved in the repair of mismatches in DNA.
Subcellular Location:
Nucleus.
Tissue Specificity:
Ubiquitous.
Subunit Structure:
Heterodimer of MLH1 and MLH3. Interacts with MTMR15/FAN1.
Family&Domains:
Belongs to the DNA mismatch repair MutL/HexB family.
Research Fields
· Genetic Information Processing > Replication and repair > Mismatch repair.
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