EXO1 Antibody - #DF3615

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Product: EXO1 Antibody
Catalog: DF3615
Description: Rabbit polyclonal antibody to EXO1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 94 KD; 94kD(Calculated).
Uniprot: Q9UQ84
RRID: AB_2835987

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
EXO1 Antibody detects endogenous levels of total EXO1.
RRID:
AB_2835987
Cite Format: Affinity Biosciences Cat# DF3615, RRID:AB_2835987.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1;

Immunogens

Immunogen:
Uniprot:

Q9UQ84(EXO1_HUMAN) >>Visit HPA database.

Gene(ID):
EXO1(9156)
Expression:
Q9UQ84 EXO1_HUMAN:

Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.

Sequence:
MGIQGLLQFIKEASEPIHVRKYKGQVVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSHGIKPILVFDGCTLPSKKEVERSRRERRQANLLKGKQLLREGKVSEARECFTRSINITHAMAHKVIKAARSQGVDCLVAPYEADAQLAYLNKAGIVQAIITEDSDLLAFGCKKVILKMDQFGNGLEIDQARLGMCRQLGDVFTEEKFRYMCILSGCDYLSSLRGIGLAKACKVLRLANNPDIVKVIKKIGHYLKMNITVPEDYINGFIRANNTFLYQLVFDPIKRKLIPLNAYEDDVDPETLSYAGQYVDDSIALQIALGNKDINTFEQIDDYNPDTAMPAHSRSHSWDDKTCQKSANVSSIWHRNYSPRPESGTVSDAPQLKENPSTVGVERVISTKGLNLPRKSSIVKRPRSAELSEDDLLSQYSLSFTKKTKKNSSEGNKSLSFSEVFVPDLVNGPTNKKSVSTPPRTRNKFATFLQRKNEESGAVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREEACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLRLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Zebrafish
91
Chicken
73
Xenopus
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQ84 As Substrate

Site PTM Type Enzyme
S68 Phosphorylation
K185 Ubiquitination
K214 Ubiquitination
S229 Phosphorylation
Y271 Phosphorylation
Y284 Phosphorylation
K292 Ubiquitination
S353 Phosphorylation
Y375 Phosphorylation
S376 Phosphorylation
K391 Ubiquitination
S404 Phosphorylation
T405 Phosphorylation
K406 Ubiquitination
S415 Phosphorylation
S422 Phosphorylation
S454 Phosphorylation
T475 Phosphorylation
T479 Phosphorylation
K482 Acetylation
K482 Sumoylation
S494 Phosphorylation
T502 Phosphorylation
S510 Phosphorylation
S512 Phosphorylation
K533 Ubiquitination
K548 Ubiquitination
T581 Phosphorylation
S598 Phosphorylation
S610 Phosphorylation
T621 Phosphorylation
S623 Phosphorylation
S639 Phosphorylation
S659 Phosphorylation
S660 Phosphorylation
S674 Phosphorylation
S700 Phosphorylation
S702 Phosphorylation
S714 Phosphorylation
T732 Phosphorylation
K743 Methylation
S746 Phosphorylation
S749 Phosphorylation
T752 Phosphorylation
K784 Sumoylation
K796 Ubiquitination
S804 Phosphorylation
S815 Phosphorylation
T824 Phosphorylation

Research Backgrounds

Function:

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.

PTMs:

Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.

Subcellular Location:

Nucleus.
Note: Colocalizes with PCNA to discrete nuclear foci in S-phase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.

Subunit Structure:

Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.

Family&Domains:

Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

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